Multiple regions of subunit interaction in Drosophila mitochondrial DNA polymerase: three functional domains in the accessory subunit.

Fan L, Kaguni LS
Biochemistry (2001), Volume 40, Page 4780
PubMed entry

Abstract:

Drosophila mitochondrial DNA polymerase, pol gamma, is a heterodimeric ...
Drosophila mitochondrial DNA polymerase, pol gamma, is a heterodimeric complex of catalytic subunit and accessory subunits. Physical interactions between the two subunits were investigated by deletion mutagenesis in both in vivo reconstitution and in vitro protein overlay analyses. Our results suggest that the accessory subunit may consist of three domains, designated the N, M, and C domains. The M and C regions comprise the major contacts involved in subunit interaction, likely with multiple sites in the exonuclease (exo) region and part of the spacer between the exo and DNA polymerase (pol) regions in the catalytic subunit. Furthermore, the N region in the accessory subunit may modulate subunit assembly and/or conformation through weak interaction with the pol region in the catalytic subunit. Sequence comparisons identify a significant similarity between the M region of the accessory subunit and the RNase H domain of HIV-1 reverse transcriptase. On the basis of these results, the proposed function of the C-terminus of the accessory subunit in RNA primer recognition, and previous observations that mitochondrial DNA polymerase is itself a reverse transcriptase, we propose that the overall conformation and arrangement of functional regions in the Drosophila pol gamma complex resemble those of HIV-1 reverse transcriptase.

Polymerases:

Topics:

Status:

new topics/pols set partial results complete validated

Results:

No results available for this paper.

Entry validated by:

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.