DNA polymerase 3 star requires ATP to start synthesis on a primed DNA.

Abstract:

DNA polymerase III star replicates a varphiX174 single-stranded, circular DNA primed with a fragment of RNA. This reaction proceeds in two stages. In stage I, a complex is formed requiring DNA polymerase III star, ATP, spermidine, copolymerase III(*), and RNA-primed varphiX174 single-stranded, circular DNA. The complex, isolated by gel filtration, contains ADP and inorganic phosphate (the products of a specific ATP cleavage) as well as spermidine, polymerase III star, and copolymerase III star. In stage II, the chain grows upon addition of deoxynucleoside triphosphates; ADP and inorganic phosphate are discharged and chain elongation is resistant to antibody to copolymerase III star. Thus ATP and copolymerase III star are required to initiate chain growth but not to sustain it.

Polymerases:

Topics:

Other Enzymatic Activities, Modulators/Inhibitors, Enzyme Substrate Interactions, Accessory Proteins/Complexes, Kinetic Parameters

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol III* Wickner W1973 Extension from RNA primer Yes
Eco Pol III* Wickner W1973 KM 8uM Reaction: Polymerase - NTP interaction; Substrate: dATP
Eco Pol III* Wickner W1973 Nucleotide incorporation accessory protein(s) copolymerase III*
Eco Pol III* Wickner W1973 Ki 400┬ÁM Reaction: Nucleotide incorporation; Inhibitor: [ATP]; Substrate: dNTPs
Eco Pol III* Wickner W1973 Percent/Fold Effect 560 fold decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Spermidine (0mM)
Eco Pol III* Wickner W1973 Percent/Fold Effect 9.33 fold decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: ATP (0mM)
Eco Pol III* Wickner W1973 Percent/Fold Effect 56 fold decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (0mM)

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