Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase.

Polymerases:

Topics:

Nucleotide Incorporation, Exonuclease Activity, Modulators/Inhibitors, Enzyme Substrate Interactions, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Jovin TM1969 3-5' Exonuclease (proofreading) Unspecified
Eco Pol I Jovin TM1969 Cloned or native Cloned in E. coli
Eco Pol I Jovin TM1969 5-3' Exonuclease Yes
Eco Pol I Jovin TM1969 Tagged Yes
Eco Pol I Jovin TM1969 Tag Name Hg(II)
Eco Pol I Jovin TM1969 Percent/Fold Effect 920 % increase Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Jovin TM1969 Percent/Fold Effect 98 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Jovin TM1969 Percent/Fold Effect 99.9 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Jovin TM1969 Percent/Fold Effect 80 % decrease Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 8.76), Temp (37°C)
Eco Pol I Jovin TM1969 Percent/Fold Effect 42 % decrease Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), Temp (37°C), pH (pH 6.5)

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