Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase.

Jovin TM, Englund PT, Kornberg A
The Journal of biological chemistry (1969), Volume 244, Page 3009
PubMed entry

Polymerases:

Eco Pol I

Topics:

Modulators/Inhibitors, Nucleotide Incorporation, Exonuclease Activity, Enzyme Substrate Interactions, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. 3-5' Exonuclease (proofreading) Unspecified
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Cloned or native Cloned in E. coli
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. 5-3' Exonuclease Yes
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Tagged Yes
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Tag Name Hg(II)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Percent/Fold Effect 920 % increase Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Percent/Fold Effect 98 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Percent/Fold Effect 99.9 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Hg(II) (1000mM), pH (pH 7.4)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Percent/Fold Effect 80 % decrease Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), pH (pH 8.76), Temp (37°C)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXVII. Chemical modifications of deoxyribonucleic acid polymerase. Percent/Fold Effect 42 % decrease Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: Hg(II) (1000mM), Temp (37°C), pH (pH 6.5)
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Entry validated by:

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Contributor: Joshua Mazzola

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Version:20240415

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