Cloning and expression of T4 DNA polymerase.

Lin TC, Rush J, Spicer EK, Konigsberg WH
Proceedings of the National Academy of Sciences of the United States of America (1987), Volume 84, Page 7000
PubMed entry Full article text

Abstract:

The structural gene coding for bacteriophage T4 DNA polymerase (gene ...
The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.

Polymerases:

T4

Topics:

Biotech Applications, Methods

One line summary:

Three-step procedure for purifying fully active T4 polymerase

Status:

new topics/pols set partial results complete validated

Results:

No results available for this paper.
Log in to add missing results...

Entry validated by:

Log in to validate this paper...

Version:20241218

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.