Cloning and expression of T4 DNA polymerase.


The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.




Biotech Applications, Methods

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Three-step procedure for purifying fully active T4 polymerase


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