Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant.
Journal of molecular biology (2002), Volume 322, Page 719
Abstract:
Polymerases:
Thermo JDF-3 pol,Taq (F667Y),Thermo JDF-3 (D141AE143A),Thermo JDF-3 (D141AE143A/P410L),Thermo JDF-3 (E143A),Thermo JDF-3 (P410LA485T)
Topics:
Mutational Analysis, Kinetic Parameters, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Exonuclease Activity
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
Thermo JDF-3 pol | Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. | 3-5' Exonuclease (proofreading) | Yes | |
Thermo JDF-3 pol | Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. | 5-3' Exonuclease | No | |
Thermo JDF-3 pol | Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. | Vmax | 2500 /minute | Reaction: Nucleotide incorporation; Substrate: dNTPs |