Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant.

Abstract:

We examined the molecular basis of ddNTP selectivity in archaeal ...

Polymerases:

Topics:

Mutational Analysis, Kinetic Parameters, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Thermo JDF-3 pol Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. 3-5' Exonuclease (proofreading) Yes
Thermo JDF-3 pol Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. 5-3' Exonuclease No
Thermo JDF-3 pol Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. Vmax 2500 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs

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