A Mutation Deleting Sequences Encoding the Amino Terminus of human Cytomegalovirus UL84 impairs Interaction with UL44 and Capsid Localization.

Protein-protein interactions are required for many biological functions. Previous work has demonstrated an interaction between the human cytomegalovirus DNA polymerase subunit UL44 and the viral replication factor UL84. In this study, glutathione-S-transferase pull down assays indicated that residues 1-68 of UL84 are both necessary and sufficient for efficient interaction of UL84 with UL44 in vitro. We created a mutant virus in which sequences encoding these residues were deleted. This mutant displayed decreased virus replication compared to wild type virus. Immunoprecipitation assays showed that the mutation decreased but did not abrogate association of UL84 with UL44 in infected cell lysate, suggesting that the interaction in the infected cell can involve other protein-protein interactions. Further immunoprecipitation assays indicated that IRS1, TRS1 or nucleolin are candidates for such interactions in infected cells. Quantitative real time PCR analysis of viral DNA indicated that the absence of the UL84 amino terminus does not notably affect viral DNA synthesis. Western blotting experiments and pulse-labeling of infected cells with [(35)S]methionine demonstrated a rather modest down regulation of levels of multiple proteins, and particularly decreased levels of the minor capsid protein UL85. Electron microscopy demonstrated that viral capsids assemble but are mislocalized in nuclei of cells infected with the mutant virus, with fewer cytoplasmic capsids detected. In sum, deletion of the sequences encoding amino terminus of UL84 affects interaction with UL44 and virus replication, but unexpectedly, not viral DNA synthesis. Mislocalization of viral capsids in infected cell nuclei likely contributes to the observed decrease in virus replication.