Involvement of Y-family DNA polymerases in mutagenesis caused by oxidized nucleotides in Escherichia coli.


Escherichia coli DNA polymerase IV incorporated 2-hydroxy-dATP ...
Escherichia coli DNA polymerase IV incorporated 2-hydroxy-dATP opposite template guanine or thymine and 8-hydroxy-dGTP exclusively opposite adenine in vitro. Mutator phenotypes in sod/fur strains were substantially diminished by deletion of dinB and/or umuDC. DNA polymerases IV and V may be involved in mutagenesis caused by incorporation of the oxidized deoxynucleoside triphosphates.




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