Results for:

Polymerase: Pfu

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Polymerase	Reference	Property	Result	Context
Pfu	High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.	3-5' Exonuclease (proofreading)	Yes
Pfu	High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.	Cloned or native	Native organism
Pfu	High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.	3-5' Exo Specific Activity	9200 units/mg
Pfu	High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.	Tagged	No
Pfu	High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.	Nucleotide Substitution Rate	1.6E-06 errors/bp
Pfu	High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.	Full length or truncated	Full length
Pfu	High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.	Specific Activity	3.171E+04 units/mg
Pfu	Incorporation of reporter-labeled nucleotides by DNA polymerases.	3-5' Exonuclease (proofreading)	Yes
Pfu	Incorporation of reporter-labeled nucleotides by DNA polymerases.	Cloned or native	Unspecified
Pfu	Incorporation of reporter-labeled nucleotides by DNA polymerases.	5-3' Exonuclease	Unspecified
Pfu	Incorporation of reporter-labeled nucleotides by DNA polymerases.	Tagged	Unspecified
Pfu	Incorporation of reporter-labeled nucleotides by DNA polymerases.	Full length or truncated	Unspecified
Pfu	Incorporation of reporter-labeled nucleotides by DNA polymerases.	Application name	PCR
Pfu	Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis	Overall Error Rate	2.18E-06 errors/bp
Pfu	Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis	V_max	21 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu	Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea.	V_max	25 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu	The 3'-5' proofreading exonuclease of archaeal family-B DNA polymerase hinders the copying of template strand deaminated bases.	3-5' Exonuclease (proofreading)	Yes
Pfu	The 3'-5' proofreading exonuclease of archaeal family-B DNA polymerase hinders the copying of template strand deaminated bases.	5-3' Exonuclease	Unspecified
Pfu	In vitro replication slippage by DNA polymerases from thermophilic organisms.	Overall Error Rate	1.3E-06 errors/bp
Pfu	In vitro replication slippage by DNA polymerases from thermophilic organisms.	Full length or truncated	Full length
Pfu	PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases.	Overall Error Rate	1.3E-06 errors/bp
Pfu	DNA polymerases from hyperthermophiles.	3-5' Exonuclease (proofreading)	Yes
Pfu	DNA polymerases from hyperthermophiles.	5-3' Exonuclease	No
Pfu	DNA polymerases from hyperthermophiles.	3-5' Exo Specific Activity	935 units/mg
Pfu	DNA polymerases from hyperthermophiles.	Overall Error Rate	1.3E-06 errors/bp
Pfu	DNA polymerases from hyperthermophiles.	Processivity	10bp
Pfu	DNA polymerases from hyperthermophiles.	Specific Activity	2.6 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Pfu	DNA polymerases from hyperthermophiles.	Specific Activity	2 units/mg	Technique: Polymerase Assay (M13 DNA)
Pfu	DNA polymerases from hyperthermophiles.	K_M	0.7nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template
Pfu	DNA polymerases from hyperthermophiles.	K_M	16uM	Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu	DNA polymerases from hyperthermophiles.	Strand Displacement	Yes
Pfu	DNA polymerases from hyperthermophiles.	k_cat	560 /minute	Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu	Interaction of the family-B DNA polymerase from the archaeon Pyrococcus furiosus with deaminated bases.	Template lesions	Stalls	Reaction: Nucleotide incorporation; Substrate: n/a; DNA lesion: dUTP (uracil)
Pfu	Interaction of the family-B DNA polymerase from the archaeon Pyrococcus furiosus with deaminated bases.	Template lesions	Stalls	Reaction: Nucleotide incorporation; Substrate: n/a; DNA lesion: dITP (inosine)
Pfu	A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement.	Overall Error Rate	1.6E-06 errors/bp	Technique: Blue/white screening (pSJ2)
Pfu	A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement.	Overall Error Rate	3.5E-06 errors/bp	Technique: Blue/white screening (pSJ3)