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Results for:

Polymerase: Pfu

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This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.

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Polymerase Reference Property Result Context
Pfu High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. 3-5' Exonuclease (proofreading) Yes
Pfu High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Cloned or native Native organism
Pfu High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. 3-5' Exo Specific Activity 9200 units/mg
Pfu High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Tagged No
Pfu High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Nucleotide Substitution Rate 1.6E-06 errors/bp
Pfu High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Full length or truncated Full length
Pfu High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Specific Activity 3.171E+04 units/mg
Pfu Incorporation of reporter-labeled nucleotides by DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Pfu Incorporation of reporter-labeled nucleotides by DNA polymerases. Cloned or native Unspecified
Pfu Incorporation of reporter-labeled nucleotides by DNA polymerases. 5-3' Exonuclease Unspecified
Pfu Incorporation of reporter-labeled nucleotides by DNA polymerases. Tagged Unspecified
Pfu Incorporation of reporter-labeled nucleotides by DNA polymerases. Full length or truncated Unspecified
Pfu Incorporation of reporter-labeled nucleotides by DNA polymerases. Application name PCR
Pfu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Overall Error Rate 2.18E-06 errors/bp
Pfu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Vmax 21 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. Vmax 25 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu The 3'-5' proofreading exonuclease of archaeal family-B DNA polymerase hinders the copying of template strand deaminated bases. 3-5' Exonuclease (proofreading) Yes
Pfu The 3'-5' proofreading exonuclease of archaeal family-B DNA polymerase hinders the copying of template strand deaminated bases. 5-3' Exonuclease Unspecified
Pfu In vitro replication slippage by DNA polymerases from thermophilic organisms. Overall Error Rate 1.3E-06 errors/bp
Pfu In vitro replication slippage by DNA polymerases from thermophilic organisms. Full length or truncated Full length
Pfu PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases. Overall Error Rate 1.3E-06 errors/bp
Pfu DNA polymerases from hyperthermophiles. 3-5' Exonuclease (proofreading) Yes
Pfu DNA polymerases from hyperthermophiles. 5-3' Exonuclease No
Pfu DNA polymerases from hyperthermophiles. 3-5' Exo Specific Activity 935 units/mg
Pfu DNA polymerases from hyperthermophiles. Overall Error Rate 1.3E-06 errors/bp
Pfu DNA polymerases from hyperthermophiles. Processivity 10bp
Pfu DNA polymerases from hyperthermophiles. Specific Activity 2.6 units/mg Technique: Polymerase Assay (calf thymus DNA)
Pfu DNA polymerases from hyperthermophiles. Specific Activity 2 units/mg Technique: Polymerase Assay (M13 DNA)
Pfu DNA polymerases from hyperthermophiles. KM 0.7nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Pfu DNA polymerases from hyperthermophiles. KM 16uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu DNA polymerases from hyperthermophiles. Strand Displacement Yes
Pfu DNA polymerases from hyperthermophiles. kcat 560 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Pfu Interaction of the family-B DNA polymerase from the archaeon Pyrococcus furiosus with deaminated bases. Template lesions Stalls Reaction: Nucleotide incorporation; Substrate: n/a; DNA lesion: dUTP (uracil)
Pfu Interaction of the family-B DNA polymerase from the archaeon Pyrococcus furiosus with deaminated bases. Template lesions Stalls Reaction: Nucleotide incorporation; Substrate: n/a; DNA lesion: dITP (inosine)
Pfu A plasmid-based lacZ╬▒ gene assay for DNA polymerase fidelity measurement. Overall Error Rate 1.6E-06 errors/bp Technique: Blue/white screening (pSJ2)
Pfu A plasmid-based lacZ╬▒ gene assay for DNA polymerase fidelity measurement. Overall Error Rate 3.5E-06 errors/bp Technique: Blue/white screening (pSJ3)

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.