show help

Results for:

Polymerase: T4 D219A

Welcome to the results summary page

This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Polymerase Reference Property Result Context
T4 D219A Hogg M2006 kcat 0.18 /second Reaction: Misincorporation; Substrate: dTTP; Technique: Multiple Turnover (across template T)
T4 D219A Hogg M2006 kcat 0.56 /second Reaction: Misincorporation; Substrate: dATP; Technique: Multiple Turnover (across template C)
T4 D219A Hogg M2006 kcat 3.36 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A Hogg M2006 kcat 402 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D219A Hogg M2006 Kd 934uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A Hogg M2006 Kd 13uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D219A Reha-Krantz LJ1993 Nucleotide Substitution Rate 500 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D219A Bhagwat M2001 RNase H Yes
T4 D219A Kroutil LC1998 Nucleotide Substitution Rate 0.0001 errors/bp Technique: M13mp2 opal codon reversion assay
T4 D219A Kroutil LC1998 Nucleotide Substitution Rate 4E-05 errors/bp Technique: M13mp2 forward mutation assay
T4 D219A Kroutil LC1998 Overall Error Rate 1.2E-05 errors/bp
T4 D219A Kroutil LC1998 5-3' Exonuclease Unspecified
T4 D219A Kroutil LC1998 3-5' Exonuclease (proofreading) No
T4 D219A Frey MW1993 kcat 3 /second Reaction: Nucleotide incorporation; Substrate: dATP
T4 D219A Frey MW1993 Kd 10uM Reaction: Nucleotide incorporation; Substrate: dATP
T4 D219A Frey MW1993 Kd 40nM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Frey MW1993 Nucleotide Substitution Rate 760 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D219A Tleugabulova D2007 3-5' Exonuclease (proofreading) No
T4 D219A Delagoutte E2003 Kd 4nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: Temp (20°C), pH (pH 7.5)
T4 D219A Delagoutte E2003 Kd 8nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: Temp (20°C), pH (pH 7.5)
T4 D219A Delagoutte E2003 Kd 95nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: Temp (20°C), pH (pH 7.5)
T4 D219A Delagoutte E2003 Kd 470nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: Temp (20°C), pH (pH 7.5)
T4 D219A Delagoutte E2003 Kd 370nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: Temp (20°C), pH (pH 7.5)
T4 D219A Delagoutte E2003 Kd 240nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: Temp (20°C), pH (pH 7.5)
T4 D219A Delagoutte E2003 Kd 24nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: Temp (20°C), pH (pH 7.5)
T4 D219A Delagoutte E2001 Vmax 55 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Delagoutte E2001 Vmax 125 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Delagoutte E2001 Vmax 0 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Delagoutte E2001 Vmax 230 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Abdus Sattar AK1996 kcat 6E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Abdus Sattar AK1996 kcat 0.096 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Abdus Sattar AK1996 KM 0.3uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Abdus Sattar AK1996 KM 12.8uM Reaction: 3-5' Exonuclease; Substrate: DNA template

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.