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Polymerase: T4 D219A

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Polymerase Reference Property Result Context
T4 D219A Probing DNA polymerase-DNA interactions: examining the template strand in exonuclease complexes using 2-aminopurine fluorescence and acrylamide quenching. 3-5' Exonuclease (proofreading) No
T4 D219A Kinetics of error generation in homologous B-family DNA polymerases. Kd 13uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D219A Kinetics of error generation in homologous B-family DNA polymerases. Kd 934uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A Kinetics of error generation in homologous B-family DNA polymerases. kcat 402 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D219A Kinetics of error generation in homologous B-family DNA polymerases. kcat 3.36 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A Kinetics of error generation in homologous B-family DNA polymerases. kcat 0.56 /second Reaction: Misincorporation; Substrate: dATP; Technique: Multiple Turnover (across template C)
T4 D219A Kinetics of error generation in homologous B-family DNA polymerases. kcat 0.18 /second Reaction: Misincorporation; Substrate: dTTP; Technique: Multiple Turnover (across template T)
T4 D219A Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. Nucleotide Substitution Rate 500 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D219A Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. Nucleotide Substitution Rate 760 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D219A Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. Kd 40nM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. Kd 10uM Reaction: Nucleotide incorporation; Substrate: dATP
T4 D219A Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. kcat 3 /second Reaction: Nucleotide incorporation; Substrate: dATP
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 12.8uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 0.3uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.096 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 6E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs. Kd 24nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs. Kd 240nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs. Kd 370nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs. Kd 470nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs. Kd 95nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs. Kd 8nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs. Kd 4nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. Vmax 230 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. Vmax 0 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. Vmax 125 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork. Vmax 55 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A Bacteriophage T4 RNase H removes both RNA primers and adjacent DNA from the 5' end of lagging strand fragments. RNase H Yes
T4 D219A Effect of accessory proteins on T4 DNA polymerase replication fidelity. 3-5' Exonuclease (proofreading) No
T4 D219A Effect of accessory proteins on T4 DNA polymerase replication fidelity. 5-3' Exonuclease Unspecified
T4 D219A Effect of accessory proteins on T4 DNA polymerase replication fidelity. Overall Error Rate 1.2E-05 errors/bp
T4 D219A Effect of accessory proteins on T4 DNA polymerase replication fidelity. Nucleotide Substitution Rate 4E-05 errors/bp Technique: M13mp2 forward mutation assay
T4 D219A Effect of accessory proteins on T4 DNA polymerase replication fidelity. Nucleotide Substitution Rate 0.0001 errors/bp Technique: M13mp2 opal codon reversion assay

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.