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Polymerase: T4 D219A

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Polymerase	Reference	Property	Result	Context
T4 D219A	Probing DNA polymerase-DNA interactions: examining the template strand in exonuclease complexes using 2-aminopurine fluorescence and acrylamide quenching.	3-5' Exonuclease (proofreading)	No
T4 D219A	Kinetics of error generation in homologous B-family DNA polymerases.	K_d	13uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D219A	Kinetics of error generation in homologous B-family DNA polymerases.	K_d	934uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A	Kinetics of error generation in homologous B-family DNA polymerases.	k_cat	402 /second	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D219A	Kinetics of error generation in homologous B-family DNA polymerases.	k_cat	3.36 /second	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D219A	Kinetics of error generation in homologous B-family DNA polymerases.	k_cat	0.56 /second	Reaction: Misincorporation; Substrate: dATP; Technique: Multiple Turnover (across template C)
T4 D219A	Kinetics of error generation in homologous B-family DNA polymerases.	k_cat	0.18 /second	Reaction: Misincorporation; Substrate: dTTP; Technique: Multiple Turnover (across template T)
T4 D219A	Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity.	Nucleotide Substitution Rate	500 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay
T4 D219A	Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.	Nucleotide Substitution Rate	760 Mutation frequency (relative to WT)	Technique: ac forward mutational assay
T4 D219A	Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.	K_d	40nM	Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A	Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.	K_d	10uM	Reaction: Nucleotide incorporation; Substrate: dATP
T4 D219A	Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.	k_cat	3 /second	Reaction: Nucleotide incorporation; Substrate: dATP
T4 D219A	Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase.	K_M	12.8uM	Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A	Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase.	K_M	0.3uM	Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A	Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase.	k_cat	0.096 /second	Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A	Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase.	k_cat	6E-05 /second	Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A	Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs.	K_d	24nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A	Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs.	K_d	240nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A	Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs.	K_d	370nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A	Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs.	K_d	470nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A	Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs.	K_d	95nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A	Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs.	K_d	8nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A	Function and assembly of the bacteriophage T4 DNA replication complex: interactions of the T4 polymerase with various model DNA constructs.	K_d	4nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template; Experimental conditions: pH (pH 7.5), Temp (20°C)
T4 D219A	Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork.	V_max	230 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A	Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork.	V_max	0 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A	Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork.	V_max	125 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A	Molecular mechanisms of the functional coupling of the helicase (gp41) and polymerase (gp43) of bacteriophage T4 within the DNA replication fork.	V_max	55 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D219A	Bacteriophage T4 RNase H removes both RNA primers and adjacent DNA from the 5' end of lagging strand fragments.	RNase H	Yes
T4 D219A	Effect of accessory proteins on T4 DNA polymerase replication fidelity.	3-5' Exonuclease (proofreading)	No
T4 D219A	Effect of accessory proteins on T4 DNA polymerase replication fidelity.	5-3' Exonuclease	Unspecified
T4 D219A	Effect of accessory proteins on T4 DNA polymerase replication fidelity.	Overall Error Rate	1.2E-05 errors/bp
T4 D219A	Effect of accessory proteins on T4 DNA polymerase replication fidelity.	Nucleotide Substitution Rate	4E-05 errors/bp	Technique: M13mp2 forward mutation assay
T4 D219A	Effect of accessory proteins on T4 DNA polymerase replication fidelity.	Nucleotide Substitution Rate	0.0001 errors/bp	Technique: M13mp2 opal codon reversion assay