Bst

From Bacillus stearothermophilus (aliases:Bst (full))
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Welcome to the Polymerase Page

This page presents all the information in Polbase for Bst.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Missing mutants?
Bst is a family A enzyme from Bacillus stearothermophilus.

Selected Properties for Bst:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
No (2) Yes (2) no data no data no data
Year Authors Title Results by property
2013 Hiromitsu Tanaka Construction of shRNA expression plas...
2010 Andrei A Golosov, Joshua J Warren, Lorena S Beese, Martin Karplus The mechanism of the translocation st...
2010 Lin Li, Jingyue Bao, Xiaoming Wu, Zhengqiang Wang, Junwei Wang, Mingxia Gong, Chunju Liu, Jinming Li Rapid detection of peste des petits r...
2009 Jennifer N Patro, Milan Urban, Robert D Kuchta Interaction of human DNA polymerase a...
2009 Michael Trostler, Alison Delier, Jeff W Beckman, Milan Urban, Jennifer N Patro, Thomas E Spratt, Lorena S Beese, Robert D Kuchta Discrimination between right and wron...
2009 Younguk Sun, Kalvin J Gregory, Val Golovlev Efficiency and specificity of microRN...
2009 Md Monsur Ali, Shuhei Imoto, Yingqian Li, Shigeki Sasaki, Fumi Nagatsugi Incorporation of an inducible nucleot...
2008 Ravindra Venkatramani, Ravi Radhakrishnan Effect of oxidatively damaged DNA on ...
2008 Linda J Reha-Krantz Recent patents of gene sequences rela...
2007 Nadezhda V Zyrina, Lyudmila A Zheleznaya, Eugene V Dvoretsky, Victor D Vasiliev, Andrei Chernov, Nicholas I Matvienko N.BspD6I DNA nickase strongly stimula...
2006 Sarit Aviel-Ronen, Chang Qi Zhu, Bradley P Coe, Ni Liu, Spencer K Watson, Wan L Lam, Ming Sound Tsao Large fragment Bst DNA polymerase for...
2005 Gerald W Hsu, Xuanwei Huang, Natalia P Luneva, Nicholas E Geacintov, Lorena S Beese Structure of a high fidelity DNA poly...
2004 Sean J Johnson, Lorena S Beese Structures of mismatch replication er...
2004 Gerald W Hsu, James R Kiefer, Dominique Y Burnouf, Olivier J Becherel, Robert P P Fuchs, Lorena S Beese Observing translesion synthesis of an...
2001 Enrique Viguera, D Canceill, S Dusko Ehrlich In vitro replication slippage by DNA ... Strand Displacement,All properties
1997 James R Kiefer, C Mao, Connie J Hansen, S L Basehore, Holly H Hogrefe, J C Braman, Lorena S Beese Crystal structure of a thermostable B...
1996 J M Aliotta, J J Pelletier, J L Ware, L S Moran, J S Benner, H Kong Thermostable Bst DNA polymerase I lac... Cloned or native,Full length or truncated,Specific Activity,3-5' exo,5-3' exo,Molecular Weight,All properties
1996 F B Perler, Sandeep Kumar, H Kong Thermostable DNA polymerases. 3-5' exo,5-3' exo,Molecular Weight,All properties
1996 M G Riggs, S Tudor, M Sivaram, S H McDonough Construction of single amino acid sub...
1995 S M Phang, C Y Teo, E Lo, V W Wong Cloning and complete sequence of the ...
1992 Y Lu, S Ye, G Hong The reverse DNA sequencing using Bst ...
1991 J McClary, S Y Ye, G F Hong, F Witney Sequencing with the large fragment of...
1991 Y Y Lu, S Y Ye, G F Hong Large fragment of DNA polymerase I fr...
1991 D A Mead, J A McClary, J A Luckey, A J Kostichka, F R Witney, L M Smith Bst DNA polymerase permits rapid sequ...
1977 J Stenesh, G R McGowan DNA polymerase from mesophilic and th... Cloned or native,Tagged,Full length or truncated,KM,All properties
Protein sequence:

Predicted Structure Model

Predicted model AlphaFold DB Not experimentally determined

This AlphaFold DB model is predicted and not an experimentally determined structure. View AlphaFold DB entry Download predicted model (.pdb)

Confidence legend (pLDDT)

AlphaFold DB colors residues by predicted confidence. Use high-confidence regions for structural context and treat low-confidence regions cautiously.

  • Very high (pLDDT > 90) Backbone and side chains are usually reliable.
  • Confident (pLDDT 70-90) Overall fold is usually reliable, but some local detail may shift.
  • Low (pLDDT 50-70) Use with caution; this region may be flexible or uncertain.
  • Very low (pLDDT < 50) Often disordered or not confidently placed.
3EZ5
3EYZ
1XC9
1UA1
1UA0
1XWL
Name 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Bst LF No (3) no consensus

Version:20260603

Using Polbase tables:

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