Klenow (exo-)

From E. coli
show help

Welcome to the Polymerase Page

This page presents all the information in Polbase for Klenow (exo-).


At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.


If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.


All references that refer to this Polymerase are listed with their topics and paper's title in the References table.


All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Klenow (exo-) is a family A enzyme from E. coli.

Selected Properties for Klenow (exo-):

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no consensus no data 1.4e-05-5.4e-04 errors/bp (2) 1.4e-05-7.6e-04 errors/bp (2) no data
Year Authors Title Results by property
2011 Chen Song, Chen Zhang, Meiping Zhao Rapid and sensitive detection of DNA ...
2011 Hirosuke Tsunoda, Tomomi Kudo, Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine Biochemical behavior of N-oxidized cy...
2010 John Chu, Marcos González-López, Scott L Cockroft, Manuel Amorin, M Reza Ghadiri Real-time monitoring of DNA polymeras...
2006 Changbei Ma, Zhiwen Tang, Kemin Wang, Weihong Tan, Jun Li, Wei Li, Zhongjie Li, Xiaohai Yang, Huimin Li, Lingfeng Liu Real-time monitoring of DNA polymeras...
2005 Alexander B Kotlyar, Natalia Borovok, Tatiana Molotsky, Dmitry Klinov, Benjamin Dwir, Eli Kapon Synthesis of novel poly(dG)-poly(dG)-... 3-5' exo,5-3' exo,All properties
2005 Alexander B Kotlyar, Natalia Borovok, Tatiana Molotsky, Ludmila Fadeev, Michael Gozin In vitro synthesis of uniform poly(dG... 3-5' exo,5-3' exo,All properties
1997 Holly Miller, Arthur P Grollman Kinetics of DNA polymerase I (Klenow ... 3-5' exo,5-3' exo,All properties
1993 Kristin A Eckert, Thomas A Kunkel Effect of reaction pH on the fidelity... Full length or truncated,Gap fill,3-5' exo,Frameshift Error Rate,Nucleotide Substitution Rate,All properties

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.