Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase.

Abstract:

We have examined as a function of pH the fidelity of DNA synthesis ...

Polymerases:

Topics:

Fidelity, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Klenow (exo-) Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. 3-5' Exonuclease (proofreading) No
Klenow (exo-) Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. Frameshift Error Rate 1.4E-05 errors/bp
Klenow (exo-) Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. Frameshift Error Rate 0.00054 errors/bp
Klenow (exo-) Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. Nucleotide Substitution Rate 1.4E-05 errors/bp Technique: Reversion (pH 6.2)
Klenow (exo-) Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. Nucleotide Substitution Rate 0.00076 errors/bp Technique: Reversion (pH 9.8)
Klenow (exo-) Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. Full length or truncated Full length
Klenow (exo-) Effect of reaction pH on the fidelity and processivity of exonuclease-deficient Klenow polymerase. Gap Filling Yes

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