Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.

Setlow P, Brutlag D, Kornberg A
The Journal of biological chemistry (1972), Volume 247, Page 224
PubMed entry

Polymerases:

Eco Pol I,Eco Pol I - Small Fragment,Klenow fragment

Topics:

Historical Protein Properties (MW, pI, ...), Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I - Small Fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Molecular Weight 3.6E+04 Dalton
Eco Pol I - Small Fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 3-5' Exonuclease (proofreading) No
Eco Pol I - Small Fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 5-3' Exonuclease Yes
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Molecular Weight 1.09E+05 Dalton
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 3-5' Exonuclease (proofreading) Yes
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 5-3' Exonuclease Yes
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Specific Activity 4500 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Extinction Coefficient 0.85 at 278nm
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Molecular Weight 7.6E+04 Dalton Technique: SDS-acrylamide gel electrophoresis
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 3-5' Exonuclease (proofreading) Yes
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. 5-3' Exonuclease No
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Specific Activity 1.534E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions. Extinction Coefficient 0.93 at 278nm
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