Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I - Small Fragment Setlow P1972 Molecular Weight 3.6E+04 Dalton
Eco Pol I - Small Fragment Setlow P1972 3-5' Exonuclease (proofreading) No
Eco Pol I - Small Fragment Setlow P1972 5-3' Exonuclease Yes
Eco Pol I Setlow P1972 Molecular Weight 1.09E+05 Dalton
Eco Pol I Setlow P1972 3-5' Exonuclease (proofreading) Yes
Eco Pol I Setlow P1972 5-3' Exonuclease Yes
Eco Pol I Setlow P1972 Specific Activity 4500 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Setlow P1972 Extinction Coefficient 0.85 at 278nm
Klenow fragment Setlow P1972 Molecular Weight 7.6E+04 Dalton Technique: SDS-acrylamide gel electrophoresis
Klenow fragment Setlow P1972 3-5' Exonuclease (proofreading) Yes
Klenow fragment Setlow P1972 5-3' Exonuclease No
Klenow fragment Setlow P1972 Specific Activity 1.534E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Setlow P1972 Extinction Coefficient 0.93 at 278nm

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