Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments.

Setlow P, Kornberg A
The Journal of biological chemistry (1972), Volume 247, Page 232
PubMed entry

Polymerases:

Eco Pol I - Small Fragment,Klenow fragment,Eco Pol I

Topics:

Historical Protein Properties (MW, pI, ...), Modulators/Inhibitors, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. Molecular Weight 7.6E+04 Dalton
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 3-5' Exonuclease (proofreading) Yes
Klenow fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 5-3' Exonuclease No
Eco Pol I - Small Fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. Molecular Weight 3.6E+04 Dalton
Eco Pol I - Small Fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 3-5' Exonuclease (proofreading) No
Eco Pol I - Small Fragment Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 5-3' Exonuclease Yes
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. Molecular Weight 1.09E+05 Dalton
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 3-5' Exonuclease (proofreading) Yes
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. 5-3' Exonuclease Yes
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. Specific Activity 4500 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. II. A proteolytic fragment containing the 5' leads to 3' exonuclease function. Restoration of intact enzyme functions from the two proteolytic fragments. Percent/Fold Effect 9.75 fold increase Reaction: 5-3' Exonuclease; Substrate: DNA template; Experimental conditions: TTP (.5mM)
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