Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching.

Abstract:

We have analyzed the kinetics of DNA synthesis catalyzed by reverse ...

Polymerases:

Topics:

Kinetic Parameters, RNase H Activity, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
HIV RT Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching. 3-5' Exonuclease (proofreading) No
HIV RT Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching. 5-3' Exonuclease No
HIV RT Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching. RNase H Yes
HIV RT Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching. Processivity 15bp
HIV RT Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching. KM 10uM Reaction: Nucleotide incorporation; Substrate: dTTP
HIV RT Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching. Kd 2.3uM Reaction: Polymerase-DNA interaction; Substrate: RNA-DNA hybrid

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