Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22.

Polymerases:

Topics:

Kinetic Parameters, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Nossal NG1971 KM 0.04uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Nossal NG1971 KM 0.08uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Nossal NG1971 Vmax 91 /minute Reaction: 3-5' Exonuclease
T4 Nossal NG1971 Vmax 2.2 /minute Reaction: 3-5' Exonuclease; Technique: Steady State (substrate: E. coli DNA, heat denatured)
T4 Q731am Nossal NG1971 KM 0.38uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Q731am Nossal NG1971 KM 0.71uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Q731am Nossal NG1971 Vmax 83 /minute Reaction: 3-5' Exonuclease
T4 Q731am Nossal NG1971 Vmax 1.1 /minute Reaction: 3-5' Exonuclease

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