Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae.

Abstract:

We have determined the fidelity of DNA synthesis by DNA polymerase I ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Fidelity, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Sce Pol I Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. Molecular Weight 1.8E+05 Dalton
Sce Pol I Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. 3-5' Exonuclease (proofreading) No
Sce Pol I Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. 5-3' Exonuclease Unspecified
Sce Pol I Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. Frameshift Error Rate 5.88E-05 errors/bp Technique: M13mp2 forward mutation assay
Sce Pol I Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. Overall Error Rate 0.000111 errors/bp Technique: M13mp2 opal codon reversion assay
Sce Pol I Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. Nucleotide Substitution Rate 8.33E-05 errors/bp Technique: M13mp2 opal codon reversion assay
Sce Pol I Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. Specific Activity 3.2E+05 units/mg

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