Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli.

Abstract:

Expression of a region of the Moloney murine leukemia virus (M-MuLV) ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), RNase H Activity, Nucleotide Incorporation, Reverse Transcriptase, Source / Purification

One line summary:

This paper shows that a gene fusion containing a portion of the bacterial trpE gene and the central portion of the M-MuLV pol gene can induce the synthesis of a stable protein with high levels of reverse transcriptase activity.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
pB6B15.23 Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. Reverse Transcriptase Activity Yes
pB6B15.23 Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. Molecular Weight 7.1E+04 Dalton Technique: SDS-PAGE
pB6B15.23 Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. Cloned or native Cloned in E. coli
pB6B15.23 Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. Tagged No
pB6B15.23 Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. Full length or truncated Full length
pB6B15.23 Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. RNase H Yes
pB6B15.23 Purification and characterization of murine retroviral reverse transcriptase expressed in Escherichia coli. Maximum Product Length 9.9kb Experimental conditions: Mg++ (8mM)

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