Fidelity of the reverse transcriptase of human immunodeficiency virus type 2.

Bakhanashvili M, Hizi A
FEBS letters (1992), Volume 306, Page 151
PubMed entry

Abstract:

The relatively low fidelity of human immunodeficiency virus type 1 ...
The relatively low fidelity of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) was implicated as a major factor that contributes to the genetic variability of the virus. Extension of mismatched 3' termini of the primer DNA was shown to be a major determinant of the infidelity of HIV-1 RT. Human immunodeficiency virus type 2 (HIV-2) also shows extensive genetic variations. Therefore, we have analyzed the fidelity of the DNA-dependent DNA polymerase activity of HIV-2 RT and compared it with those of RTs of HIV-1 and murine leukemia virus (MLV). Like other retroviral RTs, the HIV-2 RT was shown to lack a 3'----5' exonuclease activity. The ability of HIV-2 RT to extend preformed 3'-terminal A:A, A:C and A:G mispairs was examined by quantitating the amount and length of extended primers. The results demonstrate a relatively efficient mispair extension by HIV-2 RT with a specificity of A:C much greater than A:A greater than A:G. The mispair extension appears to be affected mainly by the increase of apparent Km values rather than by the change in Vmax values. The relative extension frequencies from all mispairs with HIV-1 and HIV-2 RTs was 6- to 9-fold greater than that of MLV RT, suggesting that the HIV enzymes are substantially more error-prone than MLV RT.

Polymerases:

Topics:

Exonuclease Activity, Reverse Transcriptase

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
HIV2 RT Fidelity of the reverse transcriptase of human immunodeficiency virus type 2. Reverse Transcriptase Activity Yes
HIV2 RT Fidelity of the reverse transcriptase of human immunodeficiency virus type 2. 3-5' Exonuclease (proofreading) No
HIV2 RT Fidelity of the reverse transcriptase of human immunodeficiency virus type 2. Cloned or native Cloned in E. coli
HIV RT Fidelity of the reverse transcriptase of human immunodeficiency virus type 2. Reverse Transcriptase Activity Yes
HIV RT Fidelity of the reverse transcriptase of human immunodeficiency virus type 2. 3-5' Exonuclease (proofreading) No
Klenow fragment Fidelity of the reverse transcriptase of human immunodeficiency virus type 2. Reverse Transcriptase Activity No
Klenow fragment Fidelity of the reverse transcriptase of human immunodeficiency virus type 2. 3-5' Exonuclease (proofreading) Yes

Entry validated by:

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.