A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template.
Proceedings of the National Academy of Sciences of the United States of America (1973), Volume 70, Page 1764
Abstract:
A new form of DNA polymerase III, termed Pol III star (Pol III(*)), has been purified to homogeneity from Escherichia coli. Pol III(*) is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III. Pol III(*) and Pol III are separable by gel filtration. Pol III(*) utilizes a duplex template containing short gaps with the same catalytic properties as Pol III. However, Pol III(*) is able to replicate long, singlestranded templates such as homopolymer chains and viral circles of M13 and varphiX174 if provided with the following: spermidine, a primer fragment, and a new protein, termed copolymerase III(*) (Copol III(*)). The latter, purified to homogeneity, has no known independent enzymatic activity and supports synthesis by Pol III(*) but not by Pol I, Pol II, or Pol III.
Polymerases:
Topics:
Historical Protein Properties (MW, pI, ...), Other Enzymatic Activities, Modulators/Inhibitors, Accessory Proteins/Complexes, Nucleotide Incorporation
Status:
| new | topics/pols set | partial results | complete | validated |
Results:
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| Eco Pol III* | A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. | Extension from RNA primer | Yes | |
| Eco Pol III* | A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. | Specific Activity | 1800 units/mg | |
| Eco Pol III* | A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. | Nucleotide incorporation accessory protein(s) | copolymerase III* | |
| Eco Pol III* | A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. | Percent/Fold Effect | 99 % decrease | Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Spermidine (0mM) |
| Eco Pol III* | A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. | Gap Filling | Yes |