Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase.
Biochemistry (2002), Volume 41, Page 4399
Abstract:
Polymerases:
Topics:
Other Enzymatic Activities, Biotech Applications, Kinetic Parameters, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Methods
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 D112AE114A | Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase. | Kd | 16.2uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench |
T4 D112AE114A | Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase. | Kd | 10uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench |