Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase.

Abstract:

The fluorescence of the base analogue 2-aminopurine (2AP) was used to ...

Polymerases:

Topics:

Other Enzymatic Activities, Biotech Applications, Kinetic Parameters, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Methods

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 D112AE114A Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase. Kd 16.2uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench
T4 D112AE114A Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase. Kd 10uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench

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