The dynamic processivity of the T4 DNA polymerase during replication.
Proceedings of the National Academy of Sciences of the United States of America (2004), Volume 101, Page 8289
Abstract:
Polymerases:
Topics:
Kinetic Parameters, Accessory Proteins/Complexes, Source / Purification
One line summary:
The use of mutant T4 D809N (an inactive polymerase) with wild-type polymerase shows a decrease in the rate of replication, whereby the mutant polymerase displaces the wild type polymerase. Suggests multiple polymerases may be integrated into a replisome complex during replication and may show a greater number roles in the clamp.
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 | The dynamic processivity of the T4 DNA polymerase during replication. | Full length or truncated | Full length | |
T4 | The dynamic processivity of the T4 DNA polymerase during replication. | Vmax | 150 /second | Reaction: Nucleotide incorporation; Substrate: dNTPs |
T4 | The dynamic processivity of the T4 DNA polymerase during replication. | Kd | 145nM | Reaction: Nucleotide incorporation; Substrate: dNTPs |