The dynamic processivity of the T4 DNA polymerase during replication.

Abstract:

The polymerase (gp43) processivity during T4 replisome mediated DNA ...

Polymerases:

Topics:

Kinetic Parameters, Accessory Proteins/Complexes, Source / Purification

One line summary:

The use of mutant T4 D809N (an inactive polymerase) with wild-type polymerase shows a decrease in the rate of replication, whereby the mutant polymerase displaces the wild type polymerase. Suggests multiple polymerases may be integrated into a replisome complex during replication and may show a greater number roles in the clamp.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 The dynamic processivity of the T4 DNA polymerase during replication. Full length or truncated Full length
T4 The dynamic processivity of the T4 DNA polymerase during replication. Vmax 150 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 The dynamic processivity of the T4 DNA polymerase during replication. Kd 145nM Reaction: Nucleotide incorporation; Substrate: dNTPs

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