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Polymerase: Human Pol beta

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Polymerase	Reference	Property	Result	Context
Human Pol beta	Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism.	Molecular Weight	39kD
Human Pol beta	Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism.	3-5' Exonuclease (proofreading)	No
Human Pol beta	Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism.	5-3' Exonuclease	No
Human Pol beta	Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism.	Full length or truncated	Full length
Human Pol beta	Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism.	Nick Extension	Yes, via strand displacement
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	Incorporation of non-standard nucleotides	95% +	Nucleotide analog: 2',3'-Dideoxyribonucleotide nucleoside triphosphate
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	Cloned or native	Native organism
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	Tagged	Unspecified
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	Full length or truncated	Unspecified
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	K_M	2.6uM	Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Standing start primer template)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	K_M	1.7uM	Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Standing start primer template)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	K_M	1.2uM	Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Steady State (Standing start primer template)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	K_M	2.3uM	Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Activated DNA)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	K_M	1.5uM	Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Activated DNA)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	V_max	1.5 /second	Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Standing start primer template)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	V_max	27 /second	Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Standing start primer template)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	V_max	18 /second	Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Steady State (Standing start primer template)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	Application name	PCR
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	K_d	810uM	Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Activated DNA)
Human Pol beta	Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA.	K_d	2uM	Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Steady State (Activated DNA)
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	Molecular Weight	3.9E+04 Dalton	Technique: SDS-PAGE
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	3-5' Exonuclease (proofreading)	No
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	Cloned or native	Cloned in E. coli
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	5-3' Exonuclease	No
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	Full length or truncated	Full length
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	Processivity	8bp
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	Nick Extension	No
Human Pol beta	Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme.	Gap Filling	No
Human Pol beta	Short gap-filling synthesis by DNA polymerase beta is processive.	Full length or truncated	Full length
Human Pol beta	Short gap-filling synthesis by DNA polymerase beta is processive.	Processivity	6bp
Human Pol beta	Short gap-filling synthesis by DNA polymerase beta is processive.	Gap Filling	Yes
Human Pol beta	On the processive mechanism of Escherichia coli DNA polymerase I. Quantitative assessment of processivity.	Full length or truncated	Full length
Human Pol beta	On the processive mechanism of Escherichia coli DNA polymerase I. Quantitative assessment of processivity.	Processivity	1bp
Human Pol beta	Thermostable DNA polymerases.	Molecular Weight	1.25E+05 Dalton
Human Pol beta	Thermostable DNA polymerases.	3-5' Exonuclease (proofreading)	No
Human Pol beta	Thermostable DNA polymerases.	5-3' Exonuclease	No
Human Pol beta	Thermostable DNA polymerases.	Full length or truncated	Full length
Human Pol beta	Mismatched and matched dNTP incorporation by DNA polymerase beta proceed via analogous kinetic pathways.	K_d	6.8uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (T template)
Human Pol beta	Mismatched and matched dNTP incorporation by DNA polymerase beta proceed via analogous kinetic pathways.	K_d	489uM	Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (T template)
Human Pol beta	Mismatched and matched dNTP incorporation by DNA polymerase beta proceed via analogous kinetic pathways.	K_d	2.2uM	Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (C template)
Human Pol beta	Mismatched and matched dNTP incorporation by DNA polymerase beta proceed via analogous kinetic pathways.	K_d	227uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (C template)
Human Pol beta	Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.	K_d	9uM	Reaction: Misincorporation; Substrate: dCTP; Technique: Single Turnover (G template)
Human Pol beta	Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.	K_d	590uM	Reaction: Misincorporation; Substrate: dTTP; Technique: Single Turnover (G template)
Human Pol beta	Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.	K_d	72uM	Reaction: Misincorporation; Substrate: dATP; Technique: Single Turnover (G template)
Human Pol beta	Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.	K_d	120uM	Reaction: Misincorporation; Substrate: dGTP; Technique: Single Turnover (G template)
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Molecular Weight	39kD
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	3-5' Exonuclease (proofreading)	No
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	5-3' Exonuclease	No
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Template lesions	Bypasses	Reaction: Nucleotide incorporation; Substrate: dCTP; DNA lesion: 8-oxo-dG
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Template lesions	Bypasses	Reaction: Nucleotide incorporation
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Template lesions	Bypasses	Reaction: Nucleotide incorporation
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Amino Acids Contacting Primer	Tyr271
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Overall Error Rate	0.0005 errors/bp
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Overall Error Rate	0.0024 errors/bp
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Full length or truncated	Full length
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Amino Acids Contacting Template	Arg283 and Tyr271
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Other Important Residues	Tyr271, Phe272, Asp276, and Asn279 = dNTP binding pocket; Tyr271 = important for both chemistry and dNTP binding, alters steady-state catalytic efficiency
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Amino Acids Contacting NTP	Phe272, Asp276 and Asn279
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Strand Displacement	Yes
Human Pol beta	DNA polymerase family X: function, structure, and cellular roles.	Associated condition	I260M and K289M - Cancer
Human Pol beta	The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations.	Overall Error Rate	0.076 errors/bp	Technique: M13mp2 forward mutation assay
Human Pol beta	The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations.	Gap Filling	Yes
Human Pol beta	Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene.	Nucleotide Substitution Rate	0.00067 errors/bp
Human Pol beta	Replication, Repair and Translesion Polymerase Bypass of N6-Oxopropenyl-2'-deoxyadenosine.	Template lesions	Stalls	Reaction: Nucleotide incorporation; Substrate: n/a; DNA lesion: OPdA