Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene.

Ohashi E, Bebenek K, Matsuda T, Feaver WJ, Gerlach VL, Friedberg EC, Ohmori H, Kunkel TA
The Journal of biological chemistry (2000), Volume 275, Page 39678
PubMed entry Publisher's site

Abstract:

Mammalian DNA polymerase kappa (pol kappa), a member of the UmuC/DinB ...
Mammalian DNA polymerase kappa (pol kappa), a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Here we show that human pol kappa copies undamaged DNA with average single-base substitution and deletion error rates of 7 x 10(-3) and 2 x 10(-3), respectively. These error rates are high when compared to those of most other DNA polymerases. pol kappa also has unusual error specificity, producing a high proportion of T.CMP mispairs and deleting and adding non-reiterated nucleotides at extraordinary rates. Unlike other members of the UmuC/DinB family, pol kappa can processively synthesize chains of 25 or more nucleotides. This moderate processivity may reflect a contribution of C-terminal residues, which include two zinc clusters. The very low fidelity and moderate processivity of pol kappa is novel in comparison to any previously studied DNA polymerase, and is consistent with a role in spontaneous mutagenesis.

Polymerases:

Human Pol kappa,Pol Kappa (exo-),Human Pol eta,Human Pol beta,Human Pol alpha,Human Pol delta

Topics:

Fidelity, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 3-5' Exonuclease (proofreading) No
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Cloned or native Native organism
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Tagged Yes
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Tag Name GST, His
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Nucleotide Substitution Rate 0.0058 errors/bp
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Full length or truncated Full length
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Processivity 25bp
Human Pol kappa Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Gap Filling Yes
Pol Kappa (exo-) Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. 3-5' Exonuclease (proofreading) No
Pol Kappa (exo-) Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Cloned or native Cloned in E. coli
Pol Kappa (exo-) Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Nucleotide Substitution Rate 0.0074 errors/bp
Pol Kappa (exo-) Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Full length or truncated Full length
Pol Kappa (exo-) Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Processivity 5bp
Pol Kappa (exo-) Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Gap Filling Yes
Human Pol eta Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Nucleotide Substitution Rate 0.035 errors/bp
Human Pol beta Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Nucleotide Substitution Rate 0.00067 errors/bp
Human Pol alpha Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Nucleotide Substitution Rate 0.00016 errors/bp
Human Pol delta Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Nucleotide Substitution Rate 1E-05 errors/bp
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