Pfu

From Pyrococcus furiosus
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Welcome to the Polymerase Page

This page presents all the information in Polbase for Pfu.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Missing mutants?
Pfu is a family B enzyme from Pyrococcus furiosus.

Selected Properties for Pfu:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Yes (4) No (1) no data 1.6e-06 errors/bp (1) 1.3e-06-3.5e-06 errors/bp (6)
Year Authors Title Results by property
2014 Fuming Sang, Yang Yang, Ying Lin, Zhizhou Zhang A hot start alternative for high-fide...
2013 Brian J Keith, Stanislaw K Jozwiakowski, Bernard A Connolly A plasmid-based lacZα gene assay for ... Overall Error Rate,All properties
2011 Connie J Hansen, Lydia Wu, Jeffrey D Fox, Bahram Arezi, Holly H Hogrefe Engineered split in Pfu DNA polymeras...
2011 L-P Sun, S Wang, Z-W Zhang, Y-Y Ma, Y-Q Lai, J Weng, Q-Q Zhang Interaction of gold nanoparticles wit...
2011 Xi-Peng Liu, Jian-Hua Liu Characterization of family IV UDG fro...
2010 Morten H H Nørholm A mutant Pfu DNA polymerase designed ...
2009 Suk-Tae Kwon, Hyekyung Shim, Jin Choia Jeong, Jae Kima Yun, Jae-Geun Songa, Eui-Joon Kila, Il Lee Jong Characterization and PCR optimization... Vmax,Overall Error Rate,All properties
2009 Henry J Russell, Tomas T Richardson, Kieran Emptage, Bernard A Connolly The 3'-5' proofreading exonuclease of... 3-5' exo,5-3' exo,All properties
2008 Suhng Wook Kim, Dong-Uk Kim, Jin Kwang Kim, Lin-Woo Kang, Hyun-Soo Cho Crystal structure of Pfu, the high fi...
2008 Linda J Reha-Krantz Recent patents of gene sequences rela...
2007 Hirokazu Nishida, Maiko Tanabe, Yoshizumi Ishino, Takuji Oyama, Kosuke Morikawa Crystallization and preliminary cryst...
2007 Sukhvinder Gill, Rory O'Neill, Richard J Lewis, Bernard A Connolly Interaction of the family-B DNA polym... Template lesions,All properties
2007 Nicolas Z Rudinger, Ramon Kranaster, Andreas Marx Hydrophobic amino acid and single-ato...
2006 Sotirios Melissis, Nikolaos E Labrou, Yannis D Clonis Nucleotide-mimetic synthetic ligands ...
2006 Hirokazu Nishida, Shigeki Matsumiya, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa Stoichiometric complex formation by p...
2005 Jon P Anderson, Bernhard Angerer, Lawrence A Loeb Incorporation of reporter-labeled nuc... Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Application name,All properties
2005 Yair Adereth, Kristen J Champion, Tien Hsu, Vincent Dammai Site-directed mutagenesis using Pfu D...
2005 Addendum: Site-directed mutagenesis u...
2005 Shaohui Yang, Xincang Li, Dongfeng Ding, Jianhua Hou, Zhaoxia Jin, Xinchun Yu, Tao Bo, Weidong Li, Minggang Li A method for filling in the cohesive ...
2004 G Shuttleworth, M J Fogg, MR Kurpiewski, L Jen-Jacobson, Bernard A Connolly Recognition of the pro-mutagenic base...
2004 Benjamin D Biles, Bernard A Connolly Low-fidelity Pyrococcus furiosus DNA ...
2002 Andrew F Gardner, William E Jack Acyclic and dideoxy terminator prefer...
2002 Tadayuki Imanaka, Haruyuki Atomi Catalyzing "hot" reactions: enzymes f... Vmax,All properties
2001 Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson DNA polymerases from hyperthermophiles. Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,3-5' Exo Specific Activity,Overall Error Rate,All properties
2001 Enrique Viguera, D Canceill, S Dusko Ehrlich In vitro replication slippage by DNA ... Full length or truncated,Overall Error Rate,All properties
2000 X C Li-Sucholeiki, William G Thilly A sensitive scanning technology for l...
2000 S J Evans, M J Fogg, A Mamone, M Davis, L H Pearl, Bernard A Connolly Improving dideoxynucleotide-triphosph...
2000 Kayoko Komori, Yoshizumi Ishino Functional interdependence of DNA pol...
2000 L Shao, H Shinzawa, X Zhang, D B Smith, H Watanabe, H Mitsuhashi, K Saito, T Saito, H Togashi, Tomosaburo Takahashi Diversity of hepatitis G virus within...
1998 S Goldman, R Kim, L W Hung, J Jancarik, S H Kim Purification, crystallization and pre...
1997 L A Haff, I P Smirnov Single-nucleotide polymorphism identi...
1997 Chengping Lu, H P Erickson Expression in Escherichia coli of the...
1997 P André, A Kim, Konstantin Khrapko, William G Thilly Fidelity and mutational spectrum of P...
1996 J Cline, J C Braman, Holly H Hogrefe PCR fidelity of pfu DNA polymerase an... Overall Error Rate,All properties
1995 H I Yamanaka, Y Kirii, H Ohmoto An improved phage display antibody cl...
1994 V Picard, E Ersdal-Badju, A Lu, S C Bock A rapid and efficient one-tube PCR-ba...
1994 Wayne M Barnes PCR amplification of up to 35-kb DNA ...
1992 P Forterre The DNA polymerase from the archaebac...
1991 K S Lundberg, D D Shoemaker, M W Adams, J M Short, J A Sorge, E J Mathur High-fidelity amplification using a t... Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Nucleotide Substitution Rate,3-5' Exo Specific Activity,All properties
1991 E J Mathur, M W Adams, W N Callen, J M Cline The DNA polymerase gene from the hype...
Protein sequence:

Version:20241218

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