Pfu

From Pyrococcus furiosus
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Welcome to the Polymerase Page

This page presents all the information in Polbase for Pfu.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Missing mutants?
Pfu is a family B enzyme from Pyrococcus furiosus.

Selected Properties for Pfu:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Yes (4) No (1) no data 1.6e-06 errors/bp (1) 1.3e-06-3.5e-06 errors/bp (6)
Year Authors Title Results by property
2014 Fuming Sang, Yang Yang, Ying Lin, Zhizhou Zhang A hot start alternative for high-fide...
2013 Brian J Keith, Stanislaw K Jozwiakowski, Bernard A Connolly A plasmid-based lacZα gene assay for ... Overall Error Rate,All properties
2011 Connie J Hansen, Lydia Wu, Jeffrey D Fox, Bahram Arezi, Holly H Hogrefe Engineered split in Pfu DNA polymeras...
2011 L-P Sun, S Wang, Z-W Zhang, Y-Y Ma, Y-Q Lai, J Weng, Q-Q Zhang Interaction of gold nanoparticles wit...
2011 Xi-Peng Liu, Jian-Hua Liu Characterization of family IV UDG fro...
2010 Morten H H Nørholm A mutant Pfu DNA polymerase designed ...
2009 Suk-Tae Kwon, Hyekyung Shim, Jin Choia Jeong, Jae Kima Yun, Jae-Geun Songa, Eui-Joon Kila, Il Lee Jong Characterization and PCR optimization... Vmax,Overall Error Rate,All properties
2009 Henry J Russell, Tomas T Richardson, Kieran Emptage, Bernard A Connolly The 3'-5' proofreading exonuclease of... 3-5' exo,5-3' exo,All properties
2008 Suhng Wook Kim, Dong-Uk Kim, Jin Kwang Kim, Lin-Woo Kang, Hyun-Soo Cho Crystal structure of Pfu, the high fi...
2008 Linda J Reha-Krantz Recent patents of gene sequences rela...
2007 Hirokazu Nishida, Maiko Tanabe, Yoshizumi Ishino, Takuji Oyama, Kosuke Morikawa Crystallization and preliminary cryst...
2007 Sukhvinder Gill, Rory O'Neill, Richard J Lewis, Bernard A Connolly Interaction of the family-B DNA polym... Template lesions,All properties
2007 Nicolas Z Rudinger, Ramon Kranaster, Andreas Marx Hydrophobic amino acid and single-ato...
2006 Sotirios Melissis, Nikolaos E Labrou, Yannis D Clonis Nucleotide-mimetic synthetic ligands ...
2006 Hirokazu Nishida, Shigeki Matsumiya, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa Stoichiometric complex formation by p...
2005 Jon P Anderson, Bernhard Angerer, Lawrence A Loeb Incorporation of reporter-labeled nuc... Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Application name,All properties
2005 Yair Adereth, Kristen J Champion, Tien Hsu, Vincent Dammai Site-directed mutagenesis using Pfu D...
2005 Addendum: Site-directed mutagenesis u...
2005 Shaohui Yang, Xincang Li, Dongfeng Ding, Jianhua Hou, Zhaoxia Jin, Xinchun Yu, Tao Bo, Weidong Li, Minggang Li A method for filling in the cohesive ...
2004 G Shuttleworth, M J Fogg, MR Kurpiewski, L Jen-Jacobson, Bernard A Connolly Recognition of the pro-mutagenic base...
2004 Benjamin D Biles, Bernard A Connolly Low-fidelity Pyrococcus furiosus DNA ...
2002 Andrew F Gardner, William E Jack Acyclic and dideoxy terminator prefer...
2002 Tadayuki Imanaka, Haruyuki Atomi Catalyzing "hot" reactions: enzymes f... Vmax,All properties
2001 Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson DNA polymerases from hyperthermophiles. Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,3-5' Exo Specific Activity,Overall Error Rate,All properties
2001 Enrique Viguera, D Canceill, S Dusko Ehrlich In vitro replication slippage by DNA ... Full length or truncated,Overall Error Rate,All properties
2000 X C Li-Sucholeiki, William G Thilly A sensitive scanning technology for l...
2000 S J Evans, M J Fogg, A Mamone, M Davis, L H Pearl, Bernard A Connolly Improving dideoxynucleotide-triphosph...
2000 Kayoko Komori, Yoshizumi Ishino Functional interdependence of DNA pol...
2000 L Shao, H Shinzawa, X Zhang, D B Smith, H Watanabe, H Mitsuhashi, K Saito, T Saito, H Togashi, Tomosaburo Takahashi Diversity of hepatitis G virus within...
1998 S Goldman, R Kim, L W Hung, J Jancarik, S H Kim Purification, crystallization and pre...
1997 L A Haff, I P Smirnov Single-nucleotide polymorphism identi...
1997 Chengping Lu, H P Erickson Expression in Escherichia coli of the...
1997 P André, A Kim, Konstantin Khrapko, William G Thilly Fidelity and mutational spectrum of P...
1996 J Cline, J C Braman, Holly H Hogrefe PCR fidelity of pfu DNA polymerase an... Overall Error Rate,All properties
1995 H I Yamanaka, Y Kirii, H Ohmoto An improved phage display antibody cl...
1994 V Picard, E Ersdal-Badju, A Lu, S C Bock A rapid and efficient one-tube PCR-ba...
1994 Wayne M Barnes PCR amplification of up to 35-kb DNA ...
1992 P Forterre The DNA polymerase from the archaebac...
1991 K S Lundberg, D D Shoemaker, M W Adams, J M Short, J A Sorge, E J Mathur High-fidelity amplification using a t... Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Nucleotide Substitution Rate,3-5' Exo Specific Activity,All properties
1991 E J Mathur, M W Adams, W N Callen, J M Cline The DNA polymerase gene from the hype...
Protein sequence:

Predicted Structure Model

Predicted model AlphaFold DB Not experimentally determined

This AlphaFold DB model is predicted and not an experimentally determined structure. View AlphaFold DB entry Download predicted model (.pdb)

Confidence legend (pLDDT)

AlphaFold DB colors residues by predicted confidence. Use high-confidence regions for structural context and treat low-confidence regions cautiously.

  • Very high (pLDDT > 90) Backbone and side chains are usually reliable.
  • Confident (pLDDT 70-90) Overall fold is usually reliable, but some local detail may shift.
  • Low (pLDDT 50-70) Use with caution; this region may be flexible or uncertain.
  • Very low (pLDDT < 50) Often disordered or not confidently placed.
2JGU

Version:20260603

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

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