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Polymerase: T4 A737V

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Polymerase Reference Property Result Context
T4 A737V Lo KY1976 Vmax 0.86 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A737V Lo KY1976 Vmax 1.72 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 A737V Lo KY1976 Vmax 0.52 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 A737V Lo KY1976 KM 47uM Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A737V Lo KY1976 KM 138uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 A737V Lo KY1976 KM 37uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 A737V Drake JW1993 Nucleotide Substitution Rate 0.9 Mutation frequency (relative to WT) Technique: Forward mutational (r+ -> r)
T4 A737V Spacciapoli P1994 Specific Activity 741 units/mg
T4 A737V Spacciapoli P1994 Processivity 18bp
T4 A737V Spacciapoli P1994 Full length or truncated Full length
T4 A737V Spacciapoli P1994 Cloned or native Cloned in E. coli
T4 A737V Spacciapoli P1994 3-5' Exonuclease (proofreading) Yes
T4 A737V Goodman MF1977 Nucleotide Substitution Rate 0.01 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (dCMP-AP mismatch)
T4 A737V Goodman MF1977 Nucleotide Substitution Rate 0.0025 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (AP-induced)
T4 A737V Goodman MF1977 Nucleotide Substitution Rate 0.002 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (spontaneous)
T4 A737V Reha-Krantz LJ1993 Nucleotide Substitution Rate 0.006 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A737V Wang FJ1998 Overall Error Rate 1 Mutation frequency (relative to WT) Technique: Sequencing
T4 A737V Ripley LS1983 Frameshift Error Rate 22 Mutation frequency (relative to WT) Technique: T4 rIIB N-terminal frameshift assay ((rX146; b6-mb, a + frameshift))
T4 A737V Ripley LS1983 Frameshift Error Rate 3.5 Mutation frequency (relative to WT) Technique: T4 rIIB N-terminal frameshift assay ((rFC7; b3-mb, a + frameshift))
T4 A737V Ripley LS1983 Frameshift Error Rate 6 Mutation frequency (relative to WT) Technique: T4 rIIB N-terminal frameshift assay ((rFC11; b1-mb, a + frameshift))
T4 A737V Ripley LS1983 Frameshift Error Rate 0.65 Mutation frequency (relative to WT) Technique: T4 rII 114 reversion
T4 A737V Ripley LS1983 Frameshift Error Rate 0.48 Mutation frequency (relative to WT) Technique: T4 rII UV6 reversion
T4 A737V Ripley LS1983 Frameshift Error Rate 0.13 Mutation frequency (relative to WT) Technique: T4 rII 131 reversion
T4 A737V Ripley LS1983 Frameshift Error Rate 11 Mutation frequency (relative to WT) Technique: T4 rIIB N-terminal frameshift assay ((rFC47; 1b-b7, a - frameshift))
T4 A737V Ripley LS1983 Frameshift Error Rate 1.5 Mutation frequency (relative to WT) Technique: T4 rIIB N-terminal frameshift assay ((rFC40; 1b-b4, a - frameshift))
T4 A737V Ripley LS1983 Frameshift Error Rate 4.2 Mutation frequency (relative to WT) Technique: T4 rIIB N-terminal frameshift assay ((rFC41; 1b-b2, a - frameshift))

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.