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Polymerase: T4 A737V

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Polymerase	Reference	Property	Result	Context
T4 A737V	2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.	Nucleotide Substitution Rate	0.002 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (spontaneous)
T4 A737V	2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.	Nucleotide Substitution Rate	0.0025 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (AP-induced)
T4 A737V	2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.	Nucleotide Substitution Rate	0.01 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (dCMP-AP mismatch)
T4 A737V	General antimutators are improbable.	Nucleotide Substitution Rate	0.9 Mutation frequency (relative to WT)	Technique: Forward mutational (r+ -> r)
T4 A737V	Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid.	Nucleotide Substitution Rate	0.006 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay
T4 A737V	An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.	K_M	37uM	Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 A737V	An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.	K_M	138uM	Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 A737V	An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.	K_M	47uM	Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A737V	An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.	V_max	0.52 /minute	Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 A737V	An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.	V_max	1.72 /minute	Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 A737V	An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.	V_max	0.86 /minute	Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	4.2 Mutation frequency (relative to WT)	Technique: T4 rIIB N-terminal frameshift assay ((rFC41; 1b-b2, a - frameshift))
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	1.5 Mutation frequency (relative to WT)	Technique: T4 rIIB N-terminal frameshift assay ((rFC40; 1b-b4, a - frameshift))
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	11 Mutation frequency (relative to WT)	Technique: T4 rIIB N-terminal frameshift assay ((rFC47; 1b-b7, a - frameshift))
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	0.13 Mutation frequency (relative to WT)	Technique: T4 rII 131 reversion
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	0.48 Mutation frequency (relative to WT)	Technique: T4 rII UV6 reversion
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	0.65 Mutation frequency (relative to WT)	Technique: T4 rII 114 reversion
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	6 Mutation frequency (relative to WT)	Technique: T4 rIIB N-terminal frameshift assay ((rFC11; b1-mb, a + frameshift))
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	3.5 Mutation frequency (relative to WT)	Technique: T4 rIIB N-terminal frameshift assay ((rFC7; b3-mb, a + frameshift))
T4 A737V	Mutator versus antimutator activity of a T4 DNA polymerase mutant distinguishes two different frameshifting mechanisms.	Frameshift Error Rate	22 Mutation frequency (relative to WT)	Technique: T4 rIIB N-terminal frameshift assay ((rX146; b6-mb, a + frameshift))
T4 A737V	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	3-5' Exonuclease (proofreading)	Yes
T4 A737V	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	Cloned or native	Cloned in E. coli
T4 A737V	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	Full length or truncated	Full length
T4 A737V	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	Processivity	18bp
T4 A737V	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	Specific Activity	741 units/mg
T4 A737V	The spectrum of acridine resistant mutants of bacteriophage T4 reveals cryptic effects of the tsL141 DNA polymerase allele on spontaneous mutagenesis.	Overall Error Rate	1 Mutation frequency (relative to WT)	Technique: Sequencing