DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase.

Abstract:

DNA replication in bacteria is performed by a specialized ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. Molecular Weight 1.35E+05 Dalton Technique: SDS-PAGE
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. 3-5' Exonuclease (proofreading) Yes
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. Cloned or native Cloned in E. coli
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. Tagged Yes
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. Tag Name Histidine
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. Full length or truncated Full length
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. Processivity 8.6kb
Tth Pol III DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase. Vmax 350 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (72°C)

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