Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.


The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.



Historical Protein Properties (MW, pI, ...), Nucleotide Incorporation, Exonuclease Activity, Mutational Analysis


new topics/pols set partial results complete validated


Polymerase Reference Property Result Context
Eco Pol II Kornberg T1971 3-5' Exonuclease (proofreading) Unspecified
Eco Pol II Kornberg T1971 5-3' Exonuclease Unspecified

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