Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.
Proceedings of the National Academy of Sciences of the United States of America (1971), Volume 68, Page 761
Abstract:
The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.
Polymerases:
Topics:
Mutational Analysis, Historical Protein Properties (MW, pI, ...), Nucleotide Incorporation, Exonuclease Activity
Status:
| new | topics/pols set | partial results | complete | validated |
Results:
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| Eco Pol II | Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. | 3-5' Exonuclease (proofreading) | Unspecified | |
| Eco Pol II | Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. | 5-3' Exonuclease | Unspecified |