Eco Pol II
From
E. coli
Welcome to the Polymerase Page
This page presents all the information in Polbase for Eco Pol II.
Mutants:At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.
Structures/Sequence:If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.
External links:Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.
References:All references that refer to this Polymerase are listed with their topics and paper's title in the References table.
Results:All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.
Help icons:
The symbol in the upper-right corner of the page
links to this help text. The question mark icon is used everywhere to indicate that help is available.
Eco Pol II
is a family B enzyme
from
E. coli.
Selected Properties for Eco Pol II: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Yes (3) | No (1) | no data | no data | 6.5e-04-1.5e-03 errors/bp (2) |
- References (65)
- Structures (9)
- Sequences (1)
- Mutants (1)...
- Formulations (0)
- Polymerase Lots (0)
- External links
| Year | Authors | Title | Results by property |
|---|---|---|---|
| 2013 | Richard T Pomerantz, Isabel Kurth, Myron F Goodman, Mike E O'Donnell | Preferential D-loop extension by a tr... | |
| 2013 | Christopher H Corzett, Myron F Goodman, Steven E Finkel | Competitive Fitness during Feast and ... | |
| 2013 | Chiara Indiani, Meghna Patel, Myron F Goodman, Mike E O'Donnell | RecA acts as a switch to regulate pol... | |
| 2011 | Kinrin Yamanaka, Irina G Minko, Steven E Finkel, Myron F Goodman, R Stephen Lloyd | Role of high-fidelity Escherichia col... | |
| 2010 | Mark D Sutton | Coordinating DNA polymerase traffic d... | |
| 2009 | Feng Wang, Wei Wen Yang | Structural insight into translesion s... | |
| 2009 | Karolina Makiela-Dzbenska, Malgorzata Jaszczur, Magdalena Banach-Orlowska, Piotr Jonczyk, Roel M Schaaper, I Fijalkowska | Role of Escherichia coli DNA polymera... | |
| 2008 | Anuradha Kumari, Irina G Minko, Michael B Harbut, Steven E Finkel, Myron F Goodman, R Stephen Lloyd | Replication bypass of interstrand cro... | |
| 2008 | Kaori Uchida, Asako Furukohri, Yutaka Shinozaki, Tetsuya Mori, Daichi Ogawara, Shigehiko Kanaya, Takehiko Nohmi, Hisaji Maki, Masahiro Tatsumi Akiyama | Overproduction of Escherichia coli DN... | |
| 2007 | Abu Amar M Al Mamun | Elevated expression of DNA polymerase... | |
| 2007 | Adam P Silverman, Qingfei Jiang, Myron F Goodman, Eric T Kool | Steric and electrostatic effects in D... | |
| 2006 | Abu Amar M Al Mamun, M Zafri Humayun | Escherichia coli DNA polymerase II ca... | |
| 2006 | Keiko Matsui, Masami Yamada, Masaru Imai, Kazuo Yamamoto, Takehiko Nohmi | Specificity of replicative and SOS-in... | |
| 2005 | Anna K Chikova, Roel M Schaaper | The bacteriophage P1 hot gene product... | |
| 2004 | Jacqueline M Gulbis, Steven L Kazmirski, Jeff Finkelstein, Zvi Kelman, Mike E O'Donnell, John Kuriyan | Crystal structure of the chi:psi sub-... | |
| 2004 | Eugene F DeRose, Thomas W Kirby, Geoffrey A Mueller, Anna K Chikova, Roel M Schaaper, Robert E London | Phage like it HOT: solution structure... | |
| 2002 | Zhengqiang Wang, Eli Lazarov, Mike E O'Donnell, Myron F Goodman | Resolving a fidelity paradox: why Esc... | |
| 2001 | Su-Ryang Kim, Keiko Matsui, Masami Yamada, Petr Gruz, Takehiko Nohmi | Roles of chromosomal and episomal din... | |
| 2000 | Ayelet Maor-Shoshani, N B Reuven, Guy Tomer, Zvi Livneh | Highly mutagenic replication by DNA p... | Overall Error Rate,All properties |
| 2000 | L K Savinkova, A A Sokolenko, V A Rau, T V Arshinova, Y V Gerasimova, N V Kudryashova, T S Godovikova | Affinity labeling of RNA-polymerase I... | |
| 2000 | Irina Bruck, Mike E O'Donnell | The DNA replication machine of a gram... | |
| 1999 | N B Reuven, Gali Arad, Ayelet Maor-Shoshani, Zvi Livneh | The mutagenesis protein UmuC is a DNA... | |
| 1999 | D Canceill, Enrique Viguera, S Dusko Ehrlich | Replication slippage of different DNA... | |
| 1999 | C A Clairmont, L Narayanan, Ka-Wai Sun, Peter M Glazer, Joann B Sweasy | The Tyr-265-to-Cys mutator mutant of ... | |
| 1997 | Sneha Rangarajan, G Gudmundsson, Zongxing Qiu, Patricia L Foster, Myron F Goodman | Escherichia coli DNA polymerase II ca... | |
| 1997 | T Kogoma, R R Maldonado | DNA polymerase I in constitutive stab... | |
| 1997 | Zongxing Qiu, Myron F Goodman | The Escherichia coli polB locus is id... | |
| 1996 | F B Perler, Sandeep Kumar, H Kong | Thermostable DNA polymerases. | Full length or truncated,3-5' exo,5-3' exo,Molecular Weight,All properties |
| 1995 | Patricia L Foster, G Gudmundsson, J M Trimarchi, H Cai, Myron F Goodman | Proofreading-defective DNA polymerase... | 3-5' exo,All properties |
| 1995 | H Cai, Hong Yu, K McEntee, Myron F Goodman | Purification and properties of DNA po... | |
| 1995 | H Cai, Hong Yu, K McEntee, Thomas A Kunkel, Myron F Goodman | Purification and properties of wild-t... | |
| 1994 | M Escarceller, J Hicks, G Gudmundsson, G Trump, D Touati, S Lovett, Patricia L Foster, K McEntee, Myron F Goodman | Involvement of Escherichia coli DNA p... | |
| 1994 | Yoshizumi Ishino, Hiroshi Iwasaki, Ikunoshin Kato, Hideo Shinagawa | Amino acid sequence motifs essential ... | |
| 1994 | I Tessman, M A Kennedy | DNA polymerase II of Escherichia coli... | |
| 1994 | W F Anderson, D B Prince, Hong Yu, K McEntee, Myron F Goodman | Crystallization of DNA polymerase II ... | |
| 1993 | Y W Kow, G Faundez, S Hays, C A Bonner, Myron F Goodman, Susan S Wallace | Absence of a role for DNA polymerase ... | |
| 1992 | Hiroyuki Araki, P A Ropp, A L Johnson, L H Johnston, A Morrison, Akio Sugino | DNA polymerase II, the probable homol... | |
| 1992 | Luis Blanco, Antonio Bernad, Margarita Salas | Evidence favouring the hypothesis of ... | |
| 1992 | Yoshizumi Ishino, Hiroshi Iwasaki, H Fukui, J Mineno, Ikunoshin Kato, Hideo Shinagawa | Aphidicolin inhibits DNA polymerizing... | |
| 1992 | DNA Replication | ||
| 1992 | C A Bonner, P T Stukenberg, M Rajagopalan, R Eritja, Mike E O'Donnell, K McEntee, H Echols, Myron F Goodman | Processive DNA synthesis by DNA polym... | Cloned or native,Full length or truncated,Processivity,3-5' exo,Molecular Weight,Vmax,Nucleotide incorporation accessory protein(s),All properties |
| 1991 | Hiroshi Iwasaki, Yoshizumi Ishino, H Toh, A Nakata, Hideo Shinagawa | Escherichia coli DNA polymerase II is... | |
| 1991 | A J Hughes, S K Bryan, H Chen, Robb E Moses, Charles S McHenry | Escherichia coli DNA polymerase II is... | |
| 1990 | H Chen, C B Lawrence, S K Bryan, Robb E Moses | Aphidicolin inhibits DNA polymerase I... | |
| 1990 | C A Bonner, S Hays, K McEntee, Myron F Goodman | DNA polymerase II is encoded by the D... | |
| 1989 | K C Sitney, Martin E Budd, Judith L Campbell | DNA polymerase III, a second essentia... | |
| 1989 | A Morrison, R B Christensen, J Alley, A K Beck, E G Bernstine, J F Lemontt, Christopher W Lawrence | REV3, a Saccharomyces cerevisiae gene... | |
| 1988 | G E Fradkin, E V Rabinkova, M V Torosian, O A Aĭzenberg, L A Naumova | [The role of in vivo interaction of p... | |
| 1988 | Hisaji Maki, Satoko Maki, Arthur Kornberg | DNA Polymerase III holoenzyme of Esch... | |
| 1988 | C A Bonner, S K Randall, C Rayssiguier, Miroslav Radman, R Eritja, B E Kaplan, K McEntee, Myron F Goodman | Purification and characterization of ... | |
| 1987 | Ulrich Hübscher, M Gassmann, Silvio Spadari, Neal C Brown, Elena Ferrari, H J Buhk | Mammalian DNA polymerase alpha: a rep... | |
| 1987 | Hisaji Maki, Arthur Kornberg | Proofreading by DNA polymerase III of... | |
| 1981 | O Niwa, Robb E Moses | Synthesis by DNA polymerase I on bleo... | |
| 1981 | Ulrich Hübscher, A Spanos, W Albert, F Grummt, G R Banks | Evidence that a high molecular weight... | |
| 1978 | M Włodarczyk, M Bednarska | Replication of plasmid R6K in DNA pol... | |
| 1977 | R Vicuna, Jerard Hurwitz, S Wallace, M Girard | Selective inhibition of in vitro DNA ... | |
| 1974 | K Y Ku, W H Prusoff | A comparative study of the effect of ... | |
| 1974 | Jerard Hurwitz, S Wickner | Involvement of two protein factors an... | |
| 1974 | Silvio Spadari, A Weissbach | HeLa cell R-deoxyribonucleic acid pol... | |
| 1973 | Jerard Hurwitz, S Wickner, M Wright | Studies on in vitro DNA synthesis. II... | |
| 1972 | J L Campbell, L Soll, Charles C Richardson | Isolation and partial characterizatio... | |
| 1971 | T Kornberg, M L Gefter | Purification and DNA synthesis in cel... | 3-5' exo,5-3' exo,All properties |
| 1970 | T Kornberg, M L Gefter | DNA synthesis in cell-free extracts o... | |
| 1970 | Robb E Moses, Charles C Richardson | A new DNA polymerase acitvity of Esch... | |
| 1970 | R Knippers | DNA polymerase II. |
Protein sequence:
Predicted Structure Model
Predicted model
AlphaFold DB
Not experimentally determined
This AlphaFold DB model is predicted and not an experimentally determined structure. View AlphaFold DB entry Download predicted model (.pdb)
Confidence legend (pLDDT)
AlphaFold DB colors residues by predicted confidence. Use high-confidence regions for structural context and treat low-confidence regions cautiously.
- Very high (pLDDT > 90) Backbone and side chains are usually reliable.
- Confident (pLDDT 70-90) Overall fold is usually reliable, but some local detail may shift.
- Low (pLDDT 50-70) Use with caution; this region may be flexible or uncertain.
- Very low (pLDDT < 50) Often disordered or not confidently placed.
| Name | 3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Eco Pol II exo- | No (1) |
Protein information: Uniprot P21189 search for Eco Pol II at UniProt
Sequence information: NCBI nucleotides European nucleotide archive
Predicted structure: AlphaFold DB entry AF-P21189-F1