Biochemical characterization of interactions between DNA polymerase and single-stranded DNA-binding protein in bacteriophage RB69.

Abstract:

The organization and proper assembly of proteins to the ...

Polymerases:

Topics:

Kinetic Parameters, Accessory Proteins/Complexes

One line summary:

The presence of the RB69 DNA polymerase at the primer terminus results in a 57-fold increase in gp32 (SSB) affinity for ssDNA as a result of DNA polymerase:SSB cooperativity.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
RB69 D222AD327A Biochemical characterization of interactions between DNA polymerase and single-stranded DNA-binding protein in bacteriophage RB69. Kd 124nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Technique: fluorescence titration
RB69 D222AD327A Biochemical characterization of interactions between DNA polymerase and single-stranded DNA-binding protein in bacteriophage RB69. Kd 353nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Technique: fluorescence titration (gp32 added)

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