Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells.

Abstract:

This report describes the results of our initial enzymological ...

Polymerases:

Topics:

Kinetic Parameters, Nucleotide Incorporation, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Full length or truncated Full length
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Processivity 11bp
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 44uM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 39uM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 4uM Reaction: Nucleotide incorporation; Substrate: dTTP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dCTP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 1.2uM Reaction: Nucleotide incorporation; Substrate: dGTP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 3uM Reaction: Nucleotide incorporation; Substrate: dATP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Nick Extension No
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Gap Filling Yes

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