The beta clamp targets DNA polymerase IV to DNA and strongly increases its processivity.

Abstract:

The recent discovery of a new family of ubiquitous DNA polymerases involved in translesion synthesis has shed new light onto the biochemical basis of mutagenesis. Among these polymerases, the dinB gene product (Pol IV) is involved in mutagenesis in Escherichia coli. We show here that the activity of native Pol IV is drastically modified upon interaction with the beta subunit, the processivity factor of DNA Pol III. In the absence of the beta subunit Pol IV is strictly distributive and no stable complex between Pol IV and DNA could be detected. In contrast, the beta clamp allows Pol IV to form a stable initiation complex (t 1/2 approximately 2.3 min), which leads to a dramatic increase in the processivity of PoI IV reaching an average of 300-400 nucleotides. In vivo, the beta processivity subunit may target DNA Pol IV to its substrate, generating synthesis tracks much longer than previously thought.

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Source / Purification, Nucleotide Incorporation, Kinetic Parameters, Modulators/Inhibitors

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol IV Wagner JE2000 Molecular Weight 3.95E+04 Dalton
Eco Pol IV Wagner JE2000 3-5' Exonuclease (proofreading) No
Eco Pol IV Wagner JE2000 Cloned or native Native organism
Eco Pol IV Wagner JE2000 Full length or truncated Full length
Eco Pol IV Wagner JE2000 Processivity 400bp
Eco Pol IV Wagner JE2000 Vmax 0.3 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
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