The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro.

Abstract:

We have investigated the in vitro fidelity of Escherichia coli DNA ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Fidelity, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. 3-5' Exonuclease (proofreading) Yes
Eco Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Cloned or native Native organism
Eco Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Tagged No
Eco Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Frameshift Error Rate 5E-09 errors/bp
Eco Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Nucleotide Substitution Rate 8E-08 errors/bp
Eco Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Full length or truncated Full length
Eco Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Specific Activity 3.38E+05 units/mg
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. 3-5' Exonuclease (proofreading) No
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Cloned or native Native organism
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Tagged No
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Frameshift Error Rate 5E-07 errors/bp
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Frameshift Error Rate 100 Mutation frequency (relative to WT)
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Nucleotide Substitution Rate 3.5E-06 errors/bp
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Nucleotide Substitution Rate 44 Mutation frequency (relative to WT)
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Full length or truncated Full length
MutD5 Pol III The base substitution and frameshift fidelity of Escherichia coli DNA polymerase III holoenzyme in vitro. Specific Activity 5.7E+05 units/mg

Entry validated by: