DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies.

Reha-Krantz LJ, Stocki S, Nonay RL, Dimayuga E, Goodrich LD, Konigsberg WH, Spicer EK
Proceedings of the National Academy of Sciences of the United States of America (1991), Volume 88, Page 2417
PubMed entry Full article text

Abstract:

Classical genetic selection was combined with site-directed ...

Polymerases:

T4 A89TD363N,T4,T4 D189A,T4 E191A,T4 D189AE191A,T4 E191AD324G,Eco Pol I,T4 D324G

Topics:

Mutational Analysis, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Alignments

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 D189A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 4 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D189A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D189A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 1 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 E191A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 5 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 E191A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 2 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 E191A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 6 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 D189AE191A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 16 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D189AE191A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 11 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D189AE191A DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 30 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 A89TD363N DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 74 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 36 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 D324G DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 280 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay
T4 D324G DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 63 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D324G DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 56 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 E191AD324G DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 650 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 E191AD324G DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 514 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 E191AD324G DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies. Nucleotide Substitution Rate 1800 errors/bp Technique: T4 rII P7oc reversion assay
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