In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT.

Abstract:

We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In addition, PFV RT made more deletions and insertions than HIV-1 RT. Although the majority of the missense errors made by HIV-1 RT and PFV RT are different, relatively few of the mutations caused by either enzyme can be explained by a misalignment/slippage mechanism. We suggest that the higher polymerase activity of PFV RT could contribute to the ability of the enzyme to jump to the same or a different template.

Polymerases:

Topics:

Fidelity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
HIV RT Boyer PL2007 Frameshift Error Rate 8E-06 errors/bp
HIV RT Boyer PL2007 Overall Error Rate 7.5E-05 errors/bp
HIV RT Boyer PL2007 Nucleotide Substitution Rate 6.3E-05 errors/bp
HIV RT Boyer PL2007 Full length or truncated Full length
PFV RT Boyer PL2007 Frameshift Error Rate 2.1E-05 errors/bp
PFV RT Boyer PL2007 Overall Error Rate 0.00017 errors/bp
PFV RT Boyer PL2007 Nucleotide Substitution Rate 5.8E-05 errors/bp
PFV RT Boyer PL2007 Full length or truncated Full length

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