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Hughes SH

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Publications:

Title Authors Year Journal
On the detection of multiple-binding modes of ligands to proteins, from biological, structural, and modeling data. Hughes SH Journal of computer-aided molecular design
Evaluation of natural products as inhibitors of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase. Hughes SH Journal of natural products
Mutations in HIV-1 reverse transcriptase cause misfolding and miscleavage by the viral protease. Hughes SH 2013 Virology
Effects of 4' - and 2-Substitutions on the Bioactivity of 4' -Ethynyl-2-Fluoro-2' -Deoxyadenosine. Hughes SH 2013 Antimicrobial agents and chemotherapy
A comparison of the ability of rilpivirine (TMC278) and selected analogues to inhibit clinically relevant HIV-1 reverse transcriptase mutants. Hughes SH 2012 Retrovirology
HIV-1 and HIV-2 Reverse Transcriptases: Different Mechanisms of Resistance to Nucleoside Reverse Transcriptase Inhibitors. Hughes SH 2012 Journal of virology
HIV-1 Reverse Transcription. Hughes SH 2012 Cold Spring Harbor perspectives in medicine
Foamy retrovirus integrase contains a Pol dimerization domain required for protease activation. Hughes SH 2011 Journal of virology
Mutagenesis of the human immunodeficiency virus reverse transcriptase p51 subunit defines residues contributing to vinylogous urea inhibition of Ribonuclease H activity. Hughes SH 2011 The Journal of biological chemistry
Structural basis of HIV-1 resistance to AZT by excision. Hughes SH 2010 Nature structural & molecular biology
Nature, position, and frequency of mutations made in a single cycle of HIV-1 replication. Hughes SH 2010 Journal of virology
The nucleoside analogue D-carba T blocks HIV-1 reverse transcription. Hughes SH 2009 Journal of medicinal chemistry
Structural basis for the role of the K65R mutation in HIV-1 reverse transcriptase polymerization, excision antagonism, and tenofovir resistance. Hughes SH 2009 The Journal of biological chemistry
Structure and function of HIV-1 reverse transcriptase: molecular mechanisms of polymerization and inhibition. Hughes SH 2009 Journal of molecular biology
Mutations in the thumb allow human immunodeficiency virus type 1 reverse transcriptase to be cleaved by protease in virions. Hughes SH 2009 Journal of virology
Structure of HIV-1 reverse transcriptase with the inhibitor beta-Thujaplicinol bound at the RNase H active site. Hughes SH 2009 Structure (London, England : 1993)
Apparent defects in processive DNA synthesis, strand transfer, and primer elongation of Met-184 mutants of HIV-1 reverse transcriptase derive solely from a dNTP utilization defect. Hughes SH 2008 The Journal of biological chemistry
The effects of alternate polypurine tracts (PPTs) and mutations of sequences adjacent to the PPT on viral replication and cleavage specificity of the Rous sarcoma virus reverse transcriptase. Hughes SH 2008 Journal of virology
HIV-1 reverse transcriptase connection subdomain mutations reduce template RNA degradation and enhance AZT excision. Hughes SH 2008 Proceedings of the National Academy of Sciences of the United States of America
Mutations in the U5 region adjacent to the primer binding site affect tRNA cleavage by human immunodeficiency virus type 1 reverse transcriptase in vivo. Hughes SH 2008 Journal of virology
Crystal engineering of HIV-1 reverse transcriptase for structure-based drug design. Hughes SH 2008 Nucleic acids research
High-resolution structures of HIV-1 reverse transcriptase/TMC278 complexes: strategic flexibility explains potency against resistance mutations. Hughes SH 2008 Proceedings of the National Academy of Sciences of the United States of America
In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT. Hughes SH 2007 Virology
Targeting to the endoplasmic reticulum improves the folding of recombinant human telomerase reverse transcriptase. Hughes SH 2007 Protein expression and purification
Synthesis, biological activity, and crystal structure of potent nonnucleoside inhibitors of HIV-1 reverse transcriptase that retain activity against mutant forms of the enzyme. Hughes SH 2007 Journal of medicinal chemistry
Crystal structures of clinically relevant Lys103Asn/Tyr181Cys double mutant HIV-1 reverse transcriptase in complexes with ATP and non-nucleoside inhibitor HBY 097. Hughes SH 2007 Journal of molecular biology
Analysis of amino acids in the beta7-beta8 loop of human immunodeficiency virus type 1 reverse transcriptase for their role in virus replication. Hughes SH 2007 Journal of molecular biology
The nucleoside analogs 4'C-methyl thymidine and 4'C-ethyl thymidine block DNA synthesis by wild-type HIV-1 RT and excision proficient NRTI resistant RT variants. Hughes SH 2007 Journal of molecular biology
The level of reverse transcriptase (RT) in human immunodeficiency virus type 1 particles affects susceptibility to nonnucleoside RT inhibitors but not to lamivudine. Hughes SH 2006 Journal of virology
Combining mutations in HIV-1 reverse transcriptase with mutations in the HIV-1 polypurine tract affects RNase H cleavages involved in PPT utilization. Hughes SH 2006 Virology
Why do HIV-1 and HIV-2 use different pathways to develop AZT resistance? Hughes SH 2006 PLoS Pathog
HIV-1 reverse transcriptase structure with RNase H inhibitor dihydroxy benzoyl naphthyl hydrazone bound at a novel site. Hughes SH 2006 ACS Chem Biol
In search of a novel anti-HIV drug: multidisciplinary coordination in the discovery of 4-[[4-[[4-[(1E)-2-cyanoethenyl]-2,6-dimethylphenyl]amino]-2- pyrimidinyl]amino]benzonitrile (R278474, rilpivirine). Hughes SH 2005 J Med Chem
Crystal structures for HIV-1 reverse transcriptase in complexes with three pyridinone derivatives: a new class of non-nucleoside inhibitors effective against a broad range of drug-resistant strains. Hughes SH 2005 J Med Chem
Crystallography and the design of anti-AIDS drugs: conformational flexibility and positional adaptability are important in the design of non-nucleoside HIV-1 reverse transcriptase inhibitors. Hughes SH 2005 Progress in biophysics and molecular biology
Effects of mutations in the G tract of the human immunodeficiency virus type 1 polypurine tract on virus replication and RNase H cleavage. Hughes SH 2004 J Virol
Taking aim at a moving target: designing drugs to inhibit drug-resistant HIV-1 reverse transcriptases. Hughes SH 2004 Current opinion in structural biology
A 4'-C-ethynyl-2',3'-dideoxynucleoside analogue highlights the role of the 3'-OH in anti-HIV active 4'-C-ethynyl-2'-deoxy nucleosides. Hughes SH 2004 J Med Chem
Designing anti-AIDS drugs targeting the major mechanism of HIV-1 RT resistance to nucleoside analog drugs. Hughes SH 2004 Int J Biochem Cell Biol
In vitro characterization of a simian immunodeficiency virus-human immunodeficiency virus (HIV) chimera expressing HIV type 1 reverse transcriptase to study antiviral resistance in pigtail macaques. Hughes SH 2004 Journal of virology
Characterization of the polymerase and RNase H activities of human foamy virus reverse transcriptase. Hughes SH 2004 Journal of virology
Mutations at position 184 of human immunodeficiency virus type-1 reverse transcriptase affect virus titer and viral DNA synthesis. Hughes SH 2004 Virology
PCR amplification of DNA containing non-standard base pairs by variants of reverse transcriptase from Human Immunodeficiency Virus-1. Hughes SH 2004 Nucleic acids research
Effects of the Delta67 complex of mutations in human immunodeficiency virus type 1 reverse transcriptase on nucleoside analog excision. Hughes SH 2004 J Virol
Roles of conformational and positional adaptability in structure-based design of TMC125-R165335 (etravirine) and related non-nucleoside reverse transcriptase inhibitors that are highly potent and effective against wild-type and drug-resistant HIV-1 variants. Hughes SH 2004 J Med Chem
Structures of HIV-1 RT-DNA complexes before and after incorporation of the anti-AIDS drug tenofovir. Hughes SH 2004 Nature structural & molecular biology
Nonnucleoside inhibitor binding affects the interactions of the fingers subdomain of human immunodeficiency virus type 1 reverse transcriptase with DNA. Hughes SH 2004 J Virol
Mutation of amino acids in the connection domain of human immunodeficiency virus type 1 reverse transcriptase that contact the template-primer affects RNase H activity. Hughes SH 2003 J Virol
Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer. Hughes SH 2003 J Virol
A conformationally locked analogue of the anti-HIV agent stavudine. An important correlation between pseudorotation and maximum amplitude. Hughes SH 2003 Journal of medicinal chemistry
Molecular modeling calculations of HIV-1 reverse transcriptase nonnucleoside inhibitors: correlation of binding energy with biological activity for novel 2-aryl-substituted benzimidazole analogues. Hughes SH 2003 Journal of medicinal chemistry
HIV-1 reverse transcriptase variants: molecular modeling of Y181C, V106A, L100I, and K103N mutations with nonnucleoside inhibitors using Monte Carlo simulations in combination with a linear response method. Hughes SH 2003 Drug design and discovery
Secondary structure in the nucleic acid affects the rate of HIV-1 nucleocapsid-mediated strand annealing. Hughes SH 2003 Biochemistry
Mutation of the catalytic domain of the foamy virus reverse transcriptase leads to loss of processivity and infectivity. Hughes SH 2002 Journal of virology
Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo. Hughes SH 2002 Proceedings of the National Academy of Sciences of the United States of America
Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity. Hughes SH 2002 Biochemistry
Nontemplated base addition by HIV-1 RT can induce nonspecific strand transfer in vitro. Hughes SH 2002 Virology
The M184V mutation reduces the selective excision of zidovudine 5'-monophosphate (AZTMP) by the reverse transcriptase of human immunodeficiency virus type 1. Hughes SH 2002 J Virol
Structures of HIV-1 reverse transcriptase with pre- and post-translocation AZTMP-terminated DNA. Hughes SH 2002 The EMBO journal
Nucleoside analog resistance caused by insertions in the fingers of human immunodeficiency virus type 1 reverse transcriptase involves ATP-mediated excision. Hughes SH 2002 J Virol
Nontemplated nucleotide addition by HIV-1 reverse transcriptase. Hughes SH 2002 Biochemistry
A novel mechanism for inhibition of HIV-1 reverse transcriptase. Hughes SH 2002 Bioorganic chemistry
The ribonuclease H activity of the reverse transcriptases of human immunodeficiency viruses type 1 and type 2 is affected by the thumb subdomain of the small protein subunits. Hughes SH 2001 Journal of molecular biology
Molecular matchmaking: NNRTIs can enhance the dimerization of HIV type 1 reverse transcriptase. Hughes SH 2001 Proceedings of the National Academy of Sciences of the United States of America
Replication of phenotypically mixed human immunodeficiency virus type 1 virions containing catalytically active and catalytically inactive reverse transcriptase. Hughes SH 2001 Journal of virology
The basic loop of the RNase H domain of MLV RT is important both for RNase H and for polymerase activity. Hughes SH 2001 Virology
In vitro analysis of human immunodeficiency virus type 1 minus-strand strong-stop DNA synthesis and genomic RNA processing. Hughes SH 2001 Journal of virology
The Lys103Asn mutation of HIV-1 RT: a novel mechanism of drug resistance. Hughes SH 2001 Journal of molecular biology
YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro. Hughes SH 2001 J Virol
RNase H cleavage of the 5' end of the human immunodeficiency virus type 1 genome. Hughes SH 2001 J Virol
Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA. Hughes SH 2001 The EMBO journal
Selective excision of AZTMP by drug-resistant human immunodeficiency virus reverse transcriptase. Hughes SH 2001 J Virol
Cross-linking of the fingers subdomain of human immunodeficiency virus type 1 reverse transcriptase to template-primer. Hughes SH 2001 Journal of virology
Self-priming of retroviral minus-strand strong-stop DNAs. Hughes SH 2001 Virology
Analysis of mutations at positions 115 and 116 in the dNTP binding site of HIV-1 reverse transcriptase. Hughes SH 2000 Proceedings of the National Academy of Sciences of the United States of America
Human immunodeficiency virus type 1 nucleocapsid protein can prevent self-priming of minus-strand strong stop DNA by promoting the annealing of short oligonucleotides to hairpin sequences. Hughes SH 2000 Journal of virology
Effects of amino acid substitutions at position 115 on the fidelity of human immunodeficiency virus type 1 reverse transcriptase. Hughes SH 2000 Journal of virology
The role of steric hindrance in 3TC resistance of human immunodeficiency virus type-1 reverse transcriptase. Hughes SH 2000 Journal of molecular biology
Similarities and differences in the RNase H activities of human immunodeficiency virus type 1 reverse transcriptase and Moloney murine leukemia virus reverse transcriptase. Hughes SH 1999 Journal of molecular biology
Lamivudine (3TC) resistance in HIV-1 reverse transcriptase involves steric hindrance with beta-branched amino acids. Hughes SH 1999 Proceedings of the National Academy of Sciences of the United States of America
Analysis of amino insertion mutations in the fingers subdomain of HIV-1 reverse transcriptase. Hughes SH 1999 Journal of molecular biology
Studies of the genomic RNA of leukosis viruses: implications for RNA dimerization. Hughes SH 1999 Journal of virology
Touching the heart of HIV-1 drug resistance: the fingers close down on the dNTP at the polymerase active site. Hughes SH 1999 Chemistry & biology
Structures of Tyr188Leu mutant and wild-type HIV-1 reverse transcriptase complexed with the non-nucleoside inhibitor HBY 097: inhibitor flexibility is a useful design feature for reducing drug resistance. Hughes SH 1998 Journal of molecular biology
Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 A resolution. Hughes SH 1998 Journal of molecular biology
Effects of mutations in the polymerase domain on the polymerase, RNase H and strand transfer activities of human immunodeficiency virus type 1 reverse transcriptase. Hughes SH 1998 Journal of molecular biology
Hybrid Ty1/HIV-1 elements used to detect inhibitors and monitor the activity of HIV-1 reverse transcriptase. Hughes SH 1998 Proceedings of the National Academy of Sciences of the United States of America
A mutation at position 190 of human immunodeficiency virus type 1 reverse transcriptase interacts with mutations at positions 74 and 75 via the template primer. Hughes SH 1998 Antimicrobial agents and chemotherapy
Placement of tRNA primer on the primer-binding site requires pol gene expression in avian but not murine retroviruses. Hughes SH 1997 Journal of virology
Subunit-specific mutagenesis of the cysteine 280 residue of the reverse transcriptase of human immunodeficiency virus type 1: effects on sensitivity to a specific inhibitor of the RNase H activity. Hughes SH 1997 Journal of virology
Synthesis and biological activity of novel nonnucleoside inhibitors of HIV-1 reverse transcriptase. 2-Aryl-substituted benzimidazoles. Hughes SH 1997 Journal of medicinal chemistry
Molecular modeling of HIV-1 reverse transcriptase drug-resistant mutant strains: implications for the mechanism of polymerase action. Hughes SH 1997 Protein engineering
Protein-nucleic acid interactions and DNA conformation in a complex of human immunodeficiency virus type 1 reverse transcriptase with a double-stranded DNA template-primer. Hughes SH 1997 Biopolymers
In vitro anti-HIV activity of biflavonoids isolated from Rhus succedanea and Garcinia multiflora. Hughes SH 1997 Journal of natural products
Crystal structures of 8-Cl and 9-Cl TIBO complexed with wild-type HIV-1 RT and 8-Cl TIBO complexed with the Tyr181Cys HIV-1 RT drug-resistant mutant. Hughes SH 1996 Journal of molecular biology
Specific inhibition of human immunodeficiency virus type 1 reverse transcriptase mediated by soulattrolide, a coumarin isolated from the latex of calophyllum teysmannii. Hughes SH 1996 Journal of natural products
Targeting HIV reverse transcriptase for anti-AIDS drug design: structural and biological considerations for chemotherapeutic strategies. Hughes SH 1996 Drug design and discovery
Structure of unliganded HIV-1 reverse transcriptase at 2.7 A resolution: implications of conformational changes for polymerization and inhibition mechanisms. Hughes SH 1996 Structure (London, England : 1993)
Mutagenesis in Escherichia coli by three O6-substituted guanines in double-stranded or gapped plasmids. Hughes SH 1995 Biochemistry
Crystallization of human immunodeficiency virus type 1 reverse transcriptase with and without nucleic acid substrates, inhibitors, and an antibody Fab fragment. Hughes SH 1995 Methods in enzymology
Structures of DNA and RNA polymerases and their interactions with nucleic acid substrates. Hughes SH 1995 Current opinion in structural biology
Insights into DNA polymerization mechanisms from structure and function analysis of HIV-1 reverse transcriptase. Hughes SH 1995 Biochemistry
Resistance to 1-[(2-hydroxyethoxy)methyl]-6-(phenylthio)thymine derivatives is generated by mutations at multiple sites in the HIV-1 reverse transcriptase. Hughes SH 1995 Virology
Molecular modeling studies of HIV-1 reverse transcriptase nonnucleoside inhibitors: total energy of complexation as a predictor of drug placement and activity. Hughes SH 1995 Protein science : a publication of the Protein Society
Truncating alpha-helix E' of p66 human immunodeficiency virus reverse transcriptase modulates RNase H function and impairs DNA strand transfer. Hughes SH 1995 The Journal of biological chemistry
Analysis of mutations at position 184 in reverse transcriptase of human immunodeficiency virus type 1. Hughes SH 1995 Antimicrobial agents and chemotherapy
Structure of HIV-1 RT/TIBO R 86183 complex reveals similarity in the binding of diverse nonnucleoside inhibitors. Hughes SH 1995 Nat Struct Biol
Mutational analysis of the fingers and palm subdomains of human immunodeficiency virus type-1 (HIV-1) reverse transcriptase. Hughes SH 1994 Journal of molecular biology
Sensitivity of wild-type human immunodeficiency virus type 1 reverse transcriptase to dideoxynucleotides depends on template length; the sensitivity of drug-resistant mutants does not. Hughes SH 1994 Proceedings of the National Academy of Sciences of the United States of America
The catalytic properties of the reverse transcriptase of the lentivirus equine infectious anemia virus. Hughes SH 1994 European journal of biochemistry / FEBS
Redesignation of the RNase D activity associated with retroviral reverse transcriptase as RNase H. Hughes SH 1994 Journal of virology
Enzymatic properties of two mutants of reverse transcriptase of human immunodeficiency virus type 1 (tyrosine 181-->isoleucine and tyrosine 188-->leucine), resistant to nonnucleoside inhibitors. Hughes SH 1994 AIDS research and human retroviruses
Subunit specificity of mutations that confer resistance to nonnucleoside inhibitors in human immunodeficiency virus type 1 reverse transcriptase. Hughes SH 1994 Antimicrobial agents and chemotherapy
Locations of anti-AIDS drug binding sites and resistance mutations in the three-dimensional structure of HIV-1 reverse transcriptase. Implications for mechanisms of drug inhibition and resistance. Hughes SH 1994 Journal of molecular biology
Analysis of nonnucleoside drug-resistant variants of human immunodeficiency virus type 1 reverse transcriptase. Hughes SH 1993 Journal of virology
Specific inhibition of the reverse transcriptase of human immunodeficiency virus type 1 and the chimeric enzymes of human immunodeficiency virus type 1 and type 2 by nonnucleoside inhibitors. Hughes SH 1993 Antimicrobial agents and chemotherapy
Sulfonic acid polymers are potent inhibitors of HIV-1 induced cytopathogenicity and the reverse transcriptases of both HIV-1 and HIV-2. Hughes SH 1993 Biochimica et biophysica acta
Immunization with soluble protein-pulsed spleen cells induces class I-restricted cytotoxic T lymphocytes that recognize immunodominant epitopic peptides from Plasmodium falciparum and HIV-1. Hughes SH 1992 Journal of immunology (Baltimore, Md. : 1950)
HIV-1 and HIV-2 reverse transcriptases: a comparative study of sensitivity to inhibition by selected natural products. Hughes SH 1992 Biochemical and biophysical research communications
The effects of cysteine mutations on the catalytic activities of the reverse transcriptase of human immunodeficiency virus type-1. Hughes SH 1992 The Journal of biological chemistry
Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1. Hughes SH 1992 Journal of virology
Potential anti-AIDS naphthalenesulfonic acid derivatives. Synthesis and inhibition of HIV-1 induced cytopathogenesis and HIV-1 and HIV-2 reverse transcriptase activities. Hughes SH 1992 Journal of medicinal chemistry
Mutational analysis of the fingers domain of human immunodeficiency virus type 1 reverse transcriptase. Hughes SH 1992 Journal of virology
Human immunodeficiency virus reverse transcriptase T helper epitopes identified in mice and humans: correlation with a cytotoxic T cell epitope. Hughes SH 1991 The Journal of infectious diseases
Mutational analysis of the DNA polymerase and ribonuclease H activities of human immunodeficiency virus type 2 reverse transcriptase expressed in Escherichia coli. Hughes SH 1991 Virology
Psychotrine and its O-methyl ether are selective inhibitors of human immunodeficiency virus-1 reverse transcriptase. Hughes SH 1991 The Journal of biological chemistry
The destiny of reverse transcriptase anatomy. Hughes SH 1991 Current biology : CB
Crystals of a ternary complex of human immunodeficiency virus type 1 reverse transcriptase with a monoclonal antibody Fab fragment and double-stranded DNA diffract x-rays to 3.5-A resolution. Hughes SH 1991 Proceedings of the National Academy of Sciences of the United States of America
Protein-protein interactions of HIV-1 reverse transcriptase: implication of central and C-terminal regions in subunit binding. Hughes SH 1991 Biochemistry
HIV-1 reverse transcriptase: structure predictions for the polymerase domain. Hughes SH 1990 AIDS research and human retroviruses
Immunologic and proteolytic analysis of HIV-1 reverse transcriptase structure. Hughes SH 1990 Virology
Mutational analysis of the ribonuclease H activity of human immunodeficiency virus 1 reverse transcriptase. Hughes SH 1990 Virology
Effects of small insertions on the RNA-dependent DNA polymerase activity of HIV-1 reverse transcriptase. Hughes SH 1989 Virology
Comparison of inhibitory activities of various antiretroviral agents against particle-derived and recombinant human immunodeficiency virus type 1 reverse transcriptases. Hughes SH 1989 Antimicrobial agents and chemotherapy
Expression of soluble, enzymatically active, human immunodeficiency virus reverse transcriptase in Escherichia coli and analysis of mutants. Hughes SH 1988 Proceedings of the National Academy of Sciences of the United States of America
Expression in Escherichia coli of a Moloney murine leukemia virus reverse transcriptase whose structure closely resembles the viral enzyme. Hughes SH 1988 Gene
Mapping unintegrated avian sarcoma virus DNA: termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA. Hughes SH 1978 Cell

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