DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase.
Nucleic acids research (2007), Volume 35, Page 5452
Abstract:
Polymerases:
Topics:
Biotech Applications, Kinetic Parameters, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Methods
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 W213S | DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. | Methods Featured | Fluorescence Spectroscopy | |
T4 W213S | DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. | 3-5' Exonuclease (proofreading) | No | |
T4 W213S | DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. | kcat | 1.5 /second | Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: Mg++ (8mM), Temp (37°C) |
T4 D112AE114A | DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. | 3-5' Exonuclease (proofreading) | No | |
T4 | DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. | kcat | 145 /second | Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (with heparin); Experimental conditions: Temp (37°C), Mg++ (8mM) |
T4 | DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. | kcat | 106 /second | Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Multiple Turnover (without heparin); Experimental conditions: Mg++ (8mM), Temp (37°C) |