DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase.

Abstract:

DNA polymerases achieve high-fidelity DNA replication in part by ...

Polymerases:

Topics:

Biotech Applications, Kinetic Parameters, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Methods

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 W213S DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. Methods Featured Fluorescence Spectroscopy
T4 W213S DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. 3-5' Exonuclease (proofreading) No
T4 W213S DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. kcat 1.5 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: Mg++ (8mM), Temp (37°C)
T4 D112AE114A DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. 3-5' Exonuclease (proofreading) No
T4 DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. kcat 145 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (with heparin); Experimental conditions: Temp (37°C), Mg++ (8mM)
T4 DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. kcat 106 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Multiple Turnover (without heparin); Experimental conditions: Mg++ (8mM), Temp (37°C)

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