Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases.
The Journal of biological chemistry (2002), Volume 277, Page 40640
Abstract:
Polymerases:
Topics:
Mutational Analysis, Biotech Applications, Kinetic Parameters, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Methods
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 L412M | Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. | Nucleotide Substitution Rate | 7.7 Mutation frequency (relative to WT) | Technique: rII+ reversion (2AP (1 mg/ml)) |
T4 I417V | Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. | Nucleotide Substitution Rate | 0.1 Mutation frequency (relative to WT) | Technique: rII+ reversion (2AP (1 mg/ml)) |
T4 D112AE114A | Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. | Kd | 31uM | Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (opposite template A) |