Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases.

Abstract:

The ability of wild type and mutant T4 DNA polymerases to discriminate ...

Polymerases:

Topics:

Mutational Analysis, Biotech Applications, Kinetic Parameters, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Methods

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 L412M Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. Nucleotide Substitution Rate 7.7 Mutation frequency (relative to WT) Technique: rII+ reversion (2AP (1 mg/ml))
T4 I417V Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. Nucleotide Substitution Rate 0.1 Mutation frequency (relative to WT) Technique: rII+ reversion (2AP (1 mg/ml))
T4 D112AE114A Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. Kd 31uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (opposite template A)

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