Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis.

Abstract:

Purification of DNA polymerase from E. coli B has in two cases each ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Klenow fragment Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. Molecular Weight 7E+04 Dalton Technique: Gel Filtration
Klenow fragment Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. Cloned or native Native organism
Klenow fragment Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. 5-3' Exonuclease No
Klenow fragment Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. Tagged No
Klenow fragment Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. Specific Activity 4300 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. Specific Activity 2 units/mg Technique: Exo activity (arbitrary units) (3H poly d(A-T))

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