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Polymerase: T4 A89TD363N

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Polymerase	Reference	Property	Result	Context
T4 A89TD363N	2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.	Nucleotide Substitution Rate	100 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (Spontaneous)
T4 A89TD363N	2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.	Nucleotide Substitution Rate	5 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (AP-induced)
T4 A89TD363N	2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.	Nucleotide Substitution Rate	3.8 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (dCMP-AP mismatch)
T4 A89TD363N	DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies.	Nucleotide Substitution Rate	74 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay
T4 A89TD363N	DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies.	Nucleotide Substitution Rate	36 Mutation frequency (relative to WT)	Technique: ac forward mutational assay
T4 A89TD363N	Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain.	Nucleotide Substitution Rate	20 Mutation frequency (relative to WT)	Technique: ac forward mutational assay
T4 A89TD363N	Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase.	Nucleotide Substitution Rate	80 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay
T4 A89TD363N	Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase.	Nucleotide Substitution Rate	68 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (Host: B40(sup+1))
T4 A89TD363N	Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase.	Nucleotide Substitution Rate	100 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 A89TD363N	Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase.	Nucleotide Substitution Rate	62 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (Host: B40(sup+3))
T4 A89TD363N	Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase.	Nucleotide Substitution Rate	254 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 A89TD363N	Isolation of bacteriophage T4 DNA polymerase mutator mutants.	Nucleotide Substitution Rate	90 Mutation frequency (relative to WT)	Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 A89TD363N	Isolation of bacteriophage T4 DNA polymerase mutator mutants.	Nucleotide Substitution Rate	570 Mutation frequency (relative to WT)	Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 A89TD363N	Isolation of bacteriophage T4 DNA polymerase mutator mutants.	Nucleotide Substitution Rate	1900 Mutation frequency (relative to WT)	Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 A89TD363N	Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.	V_max	790 /minute	Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A89TD363N	Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.	V_max	11 /minute	Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)