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Polymerase: T4 D112AE114A

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Polymerase	Reference	Property	Result	Context
T4 D112AE114A	DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase.	3-5' Exonuclease (proofreading)	No
T4 D112AE114A	Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases.	K_d	31uM	Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (opposite template A)
T4 D112AE114A	Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase.	K_d	16.2uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench
T4 D112AE114A	Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase.	K_d	10uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench
T4 D112AE114A	Dynamics of nucleotide incorporation: snapshots revealed by 2-aminopurine fluorescence studies.	K_d	31uM	Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (across template 2AP)
T4 D112AE114A	Kinetics of error generation in homologous B-family DNA polymerases.	K_d	11uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D112AE114A	Kinetics of error generation in homologous B-family DNA polymerases.	K_d	915uM	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D112AE114A	Kinetics of error generation in homologous B-family DNA polymerases.	k_cat	361 /second	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D112AE114A	Kinetics of error generation in homologous B-family DNA polymerases.	k_cat	3.24 /second	Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D112AE114A	Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity.	Nucleotide Substitution Rate	300 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.01 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.04 /second	Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.004 /second	Reaction: Nucleotide incorporation; Substrate: dCTP
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.03 /second	Substrate: dCTP
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.0003 /second	Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.01 /second	Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.005 /second	Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A	Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases.	V_max	0.04 /second	Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A	Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways.	Nucleotide Substitution Rate	300 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.911uM	Reaction: Nucleotide incorporation; Substrate: dCTP
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.32uM	Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A	In search of a mutational hotspot.	K_M	1.01uM	Reaction: Nucleotide incorporation; Substrate: dCTP
T4 D112AE114A	In search of a mutational hotspot.	K_M	8.24uM	Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.18uM	Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.34uM	Reaction: Misincorporation; Substrate: dATP
T4 D112AE114A	In search of a mutational hotspot.	K_M	1.38uM	Reaction: Misincorporation; Substrate: dATP
T4 D112AE114A	In search of a mutational hotspot.	K_M	8.87uM	Reaction: Misincorporation; Substrate: dATP
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.11uM	Reaction: Misincorporation; Substrate: dCTP
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.332uM	Reaction: Misincorporation; Substrate: dCTP
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.102uM	Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A	In search of a mutational hotspot.	K_M	0.683uM	Reaction: Misincorporation; Substrate: dCTP