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Polymerase: T4 D112AE114A

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Polymerase Reference Property Result Context
T4 D112AE114A DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase. 3-5' Exonuclease (proofreading) No
T4 D112AE114A Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. Nucleotide Substitution Rate 300 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D112AE114A Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases. Kd 31uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (opposite template A)
T4 D112AE114A Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase. Kd 16.2uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench
T4 D112AE114A Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase. Kd 10uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Rapid quench
T4 D112AE114A Dynamics of nucleotide incorporation: snapshots revealed by 2-aminopurine fluorescence studies. Kd 31uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (across template 2AP)
T4 D112AE114A Kinetics of error generation in homologous B-family DNA polymerases. Kd 11uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D112AE114A Kinetics of error generation in homologous B-family DNA polymerases. Kd 915uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D112AE114A Kinetics of error generation in homologous B-family DNA polymerases. kcat 361 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D112AE114A Kinetics of error generation in homologous B-family DNA polymerases. kcat 3.24 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.01 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.04 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.004 /second Reaction: Nucleotide incorporation; Substrate: dCTP
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.03 /second Substrate: dCTP
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.0003 /second Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.01 /second Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.005 /second Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A Replication of O6-methylguanine-containing DNA by repair and replicative DNA polymerases. Vmax 0.04 /second Reaction: Nucleotide incorporation; Substrate: dTTP
T4 D112AE114A Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. Nucleotide Substitution Rate 300 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D112AE114A In search of a mutational hotspot. KM 0.911uM Reaction: Nucleotide incorporation; Substrate: dCTP
T4 D112AE114A In search of a mutational hotspot. KM 0.32uM Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A In search of a mutational hotspot. KM 1.01uM Reaction: Nucleotide incorporation; Substrate: dCTP
T4 D112AE114A In search of a mutational hotspot. KM 8.24uM Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A In search of a mutational hotspot. KM 0.18uM Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A In search of a mutational hotspot. KM 0.34uM Reaction: Misincorporation; Substrate: dATP
T4 D112AE114A In search of a mutational hotspot. KM 1.38uM Reaction: Misincorporation; Substrate: dATP
T4 D112AE114A In search of a mutational hotspot. KM 8.87uM Reaction: Misincorporation; Substrate: dATP
T4 D112AE114A In search of a mutational hotspot. KM 0.11uM Reaction: Misincorporation; Substrate: dCTP
T4 D112AE114A In search of a mutational hotspot. KM 0.332uM Reaction: Misincorporation; Substrate: dCTP
T4 D112AE114A In search of a mutational hotspot. KM 0.102uM Reaction: Nucleotide incorporation; Substrate: dATP
T4 D112AE114A In search of a mutational hotspot. KM 0.683uM Reaction: Misincorporation; Substrate: dCTP

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.