show help

Results for:

Polymerase: T7

Welcome to the results summary page

This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Polymerase Reference Property Result Context
T7 Kong H1993 Processivity 1kb
T7 Kong H1993 KM 18uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
T7 Mattila P1991 3-5' Exonuclease (proofreading) Yes
T7 Mattila P1991 Frameshift Error Rate 0.0015 errors/bp Technique: Forward mutational (1mM dNTP)
T7 Mattila P1991 Nucleotide Substitution Rate 1.5E-05 errors/bp Technique: Reversion (1mM dNTP)
T7 Grippo P1971 3-5' Exonuclease (proofreading) Yes
T7 Grippo P1971 Cloned or native Native organism
T7 Grippo P1971 Tagged No
T7 Grippo P1971 Full length or truncated Full length
T7 Modrich P1975 Cloned or native Native organism
T7 Modrich P1975 Tagged No
T7 Modrich P1975 Full length or truncated Full length
T7 Modrich P1975 Specific Activity 4100 units/mg
T7 Modrich P1975 Other accessory protein(s) TsnC protein (thioredoxin)
T7 Modrich P1975 Molecular Weight 9.6E+04 Dalton Technique: SDS-PAGE (2 subunits: 84 kD gene 5 protein + 12 kD TsnC protein)
T7 Modrich P1975 Cloned or native Native organism
T7 Modrich P1975 Tagged No
T7 Modrich P1975 Full length or truncated Full length
T7 Modrich P1975 Specific Activity 1750 units/mg
T7 Hori K1979 3-5' Exonuclease (proofreading) Yes
T7 Hori K1979 Cloned or native Native organism
T7 Hori K1979 3-5' Exo Specific Activity 0.13 umol/min/mg
T7 Hori K1979 Tagged No
T7 Hori K1979 Full length or truncated Full length
T7 Hori K1979 Specific Activity 0.427 umol/min/mg
T7 Hori K1979 KM 48nM Reaction: 3-5' Exonuclease; Substrate: DNA template
T7 Hori K1979 Isoelectric Point pH 5.9
T7 Hori K1979 Other accessory protein(s) E. coli thioredoxin
T7 Nordström B1981 Molecular Weight 112kD
T7 Nordström B1981 3-5' Exonuclease (proofreading) Yes
T7 Nordström B1981 Cloned or native Cloned in E. coli
T7 Nordström B1981 3-5' Exo Specific Activity 2.4E+04 units/mg
T7 Nordström B1981 Tagged No
T7 Nordström B1981 Full length or truncated Full length
T7 Nordström B1981 Specific Activity 5150 units/mg
T7 Nordström B1981 Other accessory protein(s) E. coli thioredoxin
T7 Adler S1979 3-5' Exonuclease (proofreading) Yes
T7 Adler S1979 Cloned or native Native organism
T7 Adler S1979 3-5' Exo Specific Activity 2580 units/mg
T7 Adler S1979 Tagged No
T7 Adler S1979 Full length or truncated Full length
T7 Adler S1979 Specific Activity 3370 units/mg
T7 Adler S1979 Other accessory protein(s) E. coli thioredoxin
T7 Adler S1983 Molecular Weight 9.2E+04 Dalton
T7 Adler S1983 3-5' Exonuclease (proofreading) Yes
T7 Adler S1983 Cloned or native Native organism
T7 Adler S1983 3-5' Exo Specific Activity 9.1 umol/min/mg
T7 Adler S1983 Tagged No
T7 Adler S1983 Full length or truncated Full length
T7 Adler S1983 Specific Activity 10.7 units/mg
T7 Adler S1983 Other accessory protein(s) E. coli thioredoxin
T7 Lechner RL1983 3-5' Exonuclease (proofreading) Yes
T7 Lechner RL1983 Cloned or native Native organism
T7 Lechner RL1983 Tagged No
T7 Lechner RL1983 Full length or truncated Full length
T7 Lechner RL1983 Specific Activity 1.08E+04 units/mg
T7 Lechner RL1983 Specific Activity 5800 units/mg
T7 Lechner RL1983 Nick Extension No
T7 Lechner RL1983 Other accessory protein(s) E. coli thioredoxin
T7 Engler MJ1983 Molecular Weight 9.6E+04 Dalton
T7 Engler MJ1983 Cloned or native Cloned in E. coli
T7 Engler MJ1983 5-3' Exonuclease Yes
T7 Engler MJ1983 Tagged No
T7 Engler MJ1983 Nucleotide Substitution Rate 0.028 errors/bp
T7 Engler MJ1983 Nucleotide Substitution Rate 0.045 errors/bp
T7 Engler MJ1983 Full length or truncated Full length
T7 Engler MJ1983 Extension from RNA primer Yes
T7 Engler MJ1983 Specific Activity 5800 units/mg
T7 Engler MJ1983 Specific Activity 1.03E+04 units/mg
T7 Engler MJ1983 Nick Extension No
T7 Engler MJ1983 Other accessory protein(s) E. coli thioredoxin
T7 Patel SS1991 3-5' Exonuclease (proofreading) Yes
T7 Patel SS1991 Cloned or native Cloned in E. coli
T7 Patel SS1991 Full length or truncated Full length
T7 Patel SS1991 Processivity 1500bp
T7 Patel SS1991 KM 690nM Reaction: 3-5' Exonuclease; Substrate: DNA template
T7 Patel SS1991 Vmax 287 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Patel SS1991 Vmax 105 /second Reaction: 3-5' Exonuclease
T7 Patel SS1991 Kd 23nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
T7 Patel SS1991 Kd 18uM Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Patel SS1991 Kd 2000uM Reaction: Pyrophosphorolysis
T7 Graves SW1998 Processivity 1500bp
T7 Graves SW1998 Vmax 300 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Graves SW1998 Kd 18nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
T7 Graves SW1998 Kd 18uM Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Wuite GJ2000 3-5' Exonuclease (proofreading) Yes
T7 Wuite GJ2000 Full length or truncated Full length
T7 Wuite GJ2000 Processivity 420bp
T7 Wuite GJ2000 Vmax 100 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Tabor S1987 Cloned or native Cloned in E. coli
T7 Tabor S1987 Tagged No
T7 Tabor S1987 Full length or truncated Full length
T7 Tabor S1987 Processivity 10kb
T7 Tabor S1987 Specific Activity 8300 units/mg Technique: Polymerase Assay (calf thymus DNA)
T7 Tabor S1987 Specific Activity 5.3E+04 units/mg Technique: Polymerase Assay (M13 DNA)
T7 Tabor S1987 Vmax 100 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Bedford E1997 3-5' Exonuclease (proofreading) Yes
T7 Bedford E1997 Processivity 300bp
T7 Bedford E1997 Specific Activity 1E+04 units/mg Technique: Polymerase Assay (M13 DNA)
T7 Kunkel TA1994 3-5' Exonuclease (proofreading) Yes
T7 Kunkel TA1994 Cloned or native Native organism
T7 Kunkel TA1994 5-3' Exonuclease Yes
T7 Kunkel TA1994 Tagged No
T7 Kunkel TA1994 Frameshift Error Rate 3.7E-07 errors/bp Technique: M13mp2 forward mutation assay (+1 reading frame)
T7 Kunkel TA1994 Frameshift Error Rate 4.5E-07 errors/bp Technique: M13mp2 forward mutation assay (-1 reading frame)
T7 Kunkel TA1994 Nucleotide Substitution Rate 2.2E-06 errors/bp Technique: M13mp2 forward mutation assay
T7 Kunkel TA1994 Full length or truncated Full length
T7 Kunkel TA1994 Nucleotide incorporation accessory protein(s) Thioredoxin
T7 Yang XM1997 Full length or truncated Full length
T7 Yang XM1997 Processivity 7000bp
T7 Perler FB1996 3-5' Exonuclease (proofreading) Yes
T7 Perler FB1996 Full length or truncated Full length
T7 Perler FB1996 Vmax 700 /second Reaction: 3-5' Exonuclease
T7 Perler FB1996 Vmax 330 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Fiala KA2004 Overall Error Rate 2.6E-07 errors/bp
T7 Fiala KA2004 Overall Error Rate 6.7E-06 errors/bp
T7 Zhu B2011 Nucleotide incorporation accessory protein(s) Helicase
T7 Viikov K2011 Processivity 257bp
T7 Viikov K2011 Strand Displacement No
T7 de Vega M1999 3-5' Exonuclease (proofreading) Yes
T7 Kulczyk AW2012 Other accessory protein(s) helicase
T7 Maddukuri L2013 Template lesions Idles Reaction: Nucleotide incorporation; Substrate: dTTP; DNA lesion: OPdA

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.