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Polymerase: T7

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Polymerase Reference Property Result Context
T7 Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. Processivity 1kb
T7 Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. KM 18uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
T7 Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. 3-5' Exonuclease (proofreading) Yes
T7 Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. Frameshift Error Rate 0.0015 errors/bp Technique: Forward mutational (1mM dNTP)
T7 Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity. Nucleotide Substitution Rate 1.5E-05 errors/bp Technique: Reversion (1mM dNTP)
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. 3-5' Exonuclease (proofreading) Yes
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. Cloned or native Native organism
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. 3-5' Exo Specific Activity 0.13 umol/min/mg
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. Tagged No
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. Full length or truncated Full length
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. Specific Activity 0.427 umol/min/mg
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. KM 48nM Reaction: 3-5' Exonuclease; Substrate: DNA template
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. Isoelectric Point pH 5.9
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Purification and properties of the phage-encoded subunit, the gene 5 protein. Other accessory protein(s) E. coli thioredoxin
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. Molecular Weight 112kD
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. 3-5' Exonuclease (proofreading) Yes
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. Cloned or native Cloned in E. coli
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. 3-5' Exo Specific Activity 2.4E+04 units/mg
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. Tagged No
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. Full length or truncated Full length
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. Specific Activity 5150 units/mg
T7 Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography. Other accessory protein(s) E. coli thioredoxin
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. Molecular Weight 9.2E+04 Dalton
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. 3-5' Exonuclease (proofreading) Yes
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. Cloned or native Native organism
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. 3-5' Exo Specific Activity 9.1 umol/min/mg
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. Tagged No
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. Full length or truncated Full length
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. Specific Activity 10.7 units/mg
T7 T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups. Other accessory protein(s) E. coli thioredoxin
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. 3-5' Exonuclease (proofreading) Yes
T7 T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits. 3-5' Exonuclease (proofreading) Yes
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. 3-5' Exonuclease (proofreading) Yes
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Cloned or native Native organism
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Tagged No
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. Cloned or native Native organism
T7 Deoxyribonucleic acid polymerase of bacteriophage T7. Full length or truncated Full length
T7 Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. Cloned or native Native organism
T7 Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. Tagged No
T7 Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. Full length or truncated Full length
T7 Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. Specific Activity 4100 units/mg
T7 Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. Other accessory protein(s) TsnC protein (thioredoxin)
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. Tagged No
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. Full length or truncated Full length
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. Specific Activity 1.08E+04 units/mg
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. Specific Activity 5800 units/mg
T7 Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. Molecular Weight 9.6E+04 Dalton Technique: SDS-PAGE (2 subunits: 84 kD gene 5 protein + 12 kD TsnC protein)
T7 Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. Cloned or native Native organism
T7 Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. Tagged No
T7 Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. Full length or truncated Full length
T7 Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. Specific Activity 1750 units/mg
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. Nick Extension No
T7 Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase. Other accessory protein(s) E. coli thioredoxin
T7 Two forms of the DNA polymerase of bacteriophage T7. Molecular Weight 9.6E+04 Dalton
T7 Two forms of the DNA polymerase of bacteriophage T7. Cloned or native Cloned in E. coli
T7 Two forms of the DNA polymerase of bacteriophage T7. 5-3' Exonuclease Yes
T7 Two forms of the DNA polymerase of bacteriophage T7. Tagged No
T7 Two forms of the DNA polymerase of bacteriophage T7. Nucleotide Substitution Rate 0.028 errors/bp
T7 Two forms of the DNA polymerase of bacteriophage T7. Nucleotide Substitution Rate 0.045 errors/bp
T7 Two forms of the DNA polymerase of bacteriophage T7. Full length or truncated Full length
T7 Two forms of the DNA polymerase of bacteriophage T7. Extension from RNA primer Yes
T7 Two forms of the DNA polymerase of bacteriophage T7. Specific Activity 5800 units/mg
T7 Two forms of the DNA polymerase of bacteriophage T7. Specific Activity 1.03E+04 units/mg
T7 Two forms of the DNA polymerase of bacteriophage T7. Nick Extension No
T7 Two forms of the DNA polymerase of bacteriophage T7. Other accessory protein(s) E. coli thioredoxin
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. 3-5' Exonuclease (proofreading) Yes
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Cloned or native Cloned in E. coli
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Full length or truncated Full length
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Processivity 1500bp
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. KM 690nM Reaction: 3-5' Exonuclease; Substrate: DNA template
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Vmax 287 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Vmax 105 /second Reaction: 3-5' Exonuclease
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Kd 23nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Kd 18uM Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Kd 2000uM Reaction: Pyrophosphorolysis
T7 Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. Processivity 1500bp
T7 Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. Vmax 300 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. Kd 18nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
T7 Expression, purification, and initial kinetic characterization of the large subunit of the human mitochondrial DNA polymerase. Kd 18uM Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Single-molecule studies of the effect of template tension on T7 DNA polymerase activity. 3-5' Exonuclease (proofreading) Yes
T7 Single-molecule studies of the effect of template tension on T7 DNA polymerase activity. Full length or truncated Full length
T7 Single-molecule studies of the effect of template tension on T7 DNA polymerase activity. Processivity 420bp
T7 Single-molecule studies of the effect of template tension on T7 DNA polymerase activity. Vmax 100 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Cloned or native Cloned in E. coli
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Tagged No
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Full length or truncated Full length
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Processivity 10kb
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 8300 units/mg Technique: Polymerase Assay (calf thymus DNA)
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 5.3E+04 units/mg Technique: Polymerase Assay (M13 DNA)
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Vmax 100 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I. 3-5' Exonuclease (proofreading) Yes
T7 The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I. Processivity 300bp
T7 The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I. Specific Activity 1E+04 units/mg Technique: Polymerase Assay (M13 DNA)
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. 3-5' Exonuclease (proofreading) Yes
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Cloned or native Native organism
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. 5-3' Exonuclease Yes
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Tagged No
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Frameshift Error Rate 3.7E-07 errors/bp Technique: M13mp2 forward mutation assay (+1 reading frame)
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Frameshift Error Rate 4.5E-07 errors/bp Technique: M13mp2 forward mutation assay (-1 reading frame)
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Nucleotide Substitution Rate 2.2E-06 errors/bp Technique: M13mp2 forward mutation assay
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Full length or truncated Full length
T7 Error-prone replication of repeated DNA sequences by T7 DNA polymerase in the absence of its processivity subunit. Nucleotide incorporation accessory protein(s) Thioredoxin
T7 Amino acid changes in a unique sequence of bacteriophage T7 DNA polymerase alter the processivity of nucleotide polymerization. Full length or truncated Full length
T7 Amino acid changes in a unique sequence of bacteriophage T7 DNA polymerase alter the processivity of nucleotide polymerization. Processivity 7000bp
T7 Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
T7 Thermostable DNA polymerases. Full length or truncated Full length
T7 Thermostable DNA polymerases. Vmax 700 /second Reaction: 3-5' Exonuclease
T7 Thermostable DNA polymerases. Vmax 330 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
T7 T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits. Cloned or native Native organism
T7 T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits. 3-5' Exo Specific Activity 2580 units/mg
T7 T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits. Tagged No
T7 T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits. Full length or truncated Full length
T7 T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits. Specific Activity 3370 units/mg
T7 T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits. Other accessory protein(s) E. coli thioredoxin
T7 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Overall Error Rate 2.6E-07 errors/bp
T7 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Overall Error Rate 6.7E-06 errors/bp
T7 Bypass of a nick by the replisome of bacteriophage T7. Nucleotide incorporation accessory protein(s) Helicase
T7 Yeast mitochondrial DNA polymerase is a highly processive single-subunit enzyme. Processivity 257bp
T7 Yeast mitochondrial DNA polymerase is a highly processive single-subunit enzyme. Strand Displacement No
T7 Processive proofreading and the spatial relationship between polymerase and exonuclease active sites of bacteriophage phi29 DNA polymerase. 3-5' Exonuclease (proofreading) Yes
T7 An Interaction between DNA Polymerase and Helicase is Essential for the High Processivity of the Bacteriophage T7 Replisome. Other accessory protein(s) helicase
T7 Replication, Repair and Translesion Polymerase Bypass of N6-Oxopropenyl-2'-deoxyadenosine. Template lesions Idles Reaction: Nucleotide incorporation; Substrate: dTTP; DNA lesion: OPdA

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.