ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Modulators/Inhibitors, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Richardson CC1964 3-5' Exonuclease (proofreading) Unspecified
Eco Pol I Richardson CC1964 5-3' Exonuclease Unspecified
Eco Pol I Richardson CC1964 Optimal pH pH 7.4
Eco Pol I Richardson CC1964 Specific Activity 1.88E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Richardson CC1964 Percent/Fold Effect 30 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: pH (pH 7)
Eco Pol I Richardson CC1964 Percent/Fold Effect 30 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: pH (pH 7.8)
Eco Pol I Exonuclease II Richardson CC1964 3-5' Exonuclease (proofreading) Yes
Eco Pol I Exonuclease II Richardson CC1964 Optimal pH pH 9
Bsu Richardson CC1964 3-5' Exonuclease (proofreading) No
Bsu Richardson CC1964 5-3' Exonuclease No
Bsu Richardson CC1964 Optimal pH pH 7.4
Bsu Richardson CC1964 Percent/Fold Effect 0 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (7mM)
Bsu Richardson CC1964 Percent/Fold Effect 70 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (.33mM)
Bsu Richardson CC1964 Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (33mM)

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