The human DINB1 gene encodes the DNA polymerase Poltheta.

Abstract:

The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) theta. hPoltheta is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6-4) photoproduct or an abasic site. We also examine the fidelity of hPoltheta on nondamaged DNA templates by steady-state kinetic analyses and find that hPoltheta misincorporates deoxynucleotides with a frequency of about 10(-3) to 10(-4). We discuss the relationship between the fidelity of hPoltheta and its inability to bypass DNA damage.

Polymerases:

Topics:

Exonuclease Activity, Source / Purification, Nucleotide Analogs / Template Lesions, Fidelity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol theta Johnson RE2000 3-5' Exonuclease (proofreading) No
Human Pol theta Johnson RE2000 Cloned or native Cloned in yeast
Human Pol theta Johnson RE2000 Template lesions Stalls Reaction: Nucleotide incorporation; DNA lesion: TT Cyclobutane Pyrimidine Dimer
Human Pol theta Johnson RE2000 Template lesions Stalls Reaction: Nucleotide incorporation; DNA lesion: TC 6,4 Photodimer
Human Pol theta Johnson RE2000 Template lesions Stalls Reaction: Nucleotide incorporation; DNA lesion: Apurinic/Apyrimidinic (AP) site
Human Pol theta Johnson RE2000 Tagged Yes
Human Pol theta Johnson RE2000 Tag Name GST
Human Pol theta Johnson RE2000 Overall Error Rate 0.01 errors/bp
Human Pol theta Johnson RE2000 Overall Error Rate 0.001 errors/bp

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