Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.

Bebenek A, Dressman HK, Carver GT, Ng S, Petrov VM, Yang G, Konigsberg WH, Karam JD, Drake JW

The Journal of biological chemistry (2001), Volume 276, Page 10387

PubMed entry Publisher's site

Abstract:

The DNA polymerases (gp43s) of the related bacteriophages T4 and RB69 ...

Polymerases:

RB69 Y567A,RB69,RB69 Y567S,RB69 Y567T,RB69 D222AD327A/Y567A,RB69 D222AD327A

Topics:

Kinetic Parameters, Fidelity, Nucleotide Incorporation, Exonuclease Activity

One line summary:

In RB69 polymerase Tyrosine 567 is an integral part of base selection, as RB69 Y567A is less accurate and slower at replicating than an RB69 mutant without exonuclease capabilities

Status:

new	topics/pols set	partial results	complete	validated

Results:

Polymerase	Reference	Property	Result	Context
RB69 Y567A	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Frameshift Error Rate	4.5 Mutation frequency (relative to WT)	Technique: RB69 rII 131 reversion
RB69 Y567A	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	820 Mutation frequency (relative to WT)	Technique: RB69 rII UV256 reversion
RB69 Y567A	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	1400 Mutation frequency (relative to WT)	Technique: RB69 rII UV375 reversion
RB69 Y567S	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Frameshift Error Rate	14 Mutation frequency (relative to WT)	Technique: RB69 rII 131 reversion
RB69 Y567S	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	1200 Mutation frequency (relative to WT)	Technique: RB69 rII UV256 reversion
RB69 Y567S	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	1600 Mutation frequency (relative to WT)	Technique: RB69 rII UV375 reversion
RB69 Y567T	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Frameshift Error Rate	32 Mutation frequency (relative to WT)	Technique: RB69 rII 131 reversion
RB69 Y567T	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	2600 Mutation frequency (relative to WT)	Technique: RB69 rII UV256 reversion
RB69 Y567T	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	810 Mutation frequency (relative to WT)	Technique: RB69 rII UV375 reversion
RB69 D222AD327A	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Frameshift Error Rate	520 Mutation frequency (relative to WT)	Technique: RB69 rII 131 reversion
RB69 D222AD327A	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	600 Mutation frequency (relative to WT)	Technique: RB69 rII UV256 reversion
RB69 D222AD327A	Interacting fidelity defects in the replicative DNA polymerase of bacteriophage RB69.	Nucleotide Substitution Rate	2600 Mutation frequency (relative to WT)	Technique: RB69 rII UV375 reversion