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Polymerase: Human Pol alpha

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This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.

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Polymerase Reference Property Result Context
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. KM 0.2uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. KM 89uM Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. Vmax 1.1 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. Vmax 1.7 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Human Pol alpha Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases. 3-5' Exonuclease (proofreading) No
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Full length or truncated Full length
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Processivity 11bp
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 44uM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 39uM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 4uM Reaction: Nucleotide incorporation; Substrate: dTTP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dCTP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 1.2uM Reaction: Nucleotide incorporation; Substrate: dGTP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. KM 3uM Reaction: Nucleotide incorporation; Substrate: dATP
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Nick Extension No
Human Pol alpha Enzymological characterization of DNA polymerase alpha. Basic catalytic properties processivity, and gap utilization of the homogeneous enzyme from human KB cells. Gap Filling Yes
Human Pol alpha Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. 3-5' Exonuclease (proofreading) No
Human Pol alpha Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. 5-3' Exonuclease No
Human Pol alpha Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. RNase H No
Human Pol alpha Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. Processivity 450bp
Human Pol alpha Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. Specific Activity 490 units/mg Technique: Polymerase Assay (M13 DNA)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Incorporation of non-standard nucleotides < 10% Nucleotide analog: Acycloriboucleoside-5'-O-(1-Thiotriphosphate)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Incorporation of non-standard nucleotides Unspecified Nucleotide analog: 2',3'-Dideoxyribonucleotide nucleoside triphosphate
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Cloned or native Native organism
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Tagged Unspecified
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Full length or truncated Unspecified
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. KM 1.5uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Activated DNA)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. KM 1.8uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (activated DNA)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. KM 110uM Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Running start primer template)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. KM 1.4uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Running start primer template)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. KM 2.1uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Running start primer template)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Vmax 2 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Running start primer template)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Vmax 0.06 /second Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Running start primer template)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Vmax 1.7 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Running start primer template)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Application name PCR
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Kd 120uM Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Steady State (Activated DNA)
Human Pol alpha Human DNA polymerases alpha and beta are able to incorporate anti-HIV deoxynucleotides into DNA. Kd 650uM Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Activated DNA)
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. KM 3.7uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. KM 4200uM Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. KM 1E+04uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. KM 1E+04uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. KM 35uM Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. Vmax 12.9 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. Vmax 3.1 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. Vmax 1.7 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. Vmax 1.7 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Gel shift
Human Pol alpha DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. Vmax 13 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Gel shift
Human Pol alpha Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) No
Human Pol alpha Thermostable DNA polymerases. 5-3' Exonuclease No
Human Pol alpha Thermostable DNA polymerases. Full length or truncated Full length
Human Pol alpha Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. 3-5' Exonuclease (proofreading) No
Human Pol alpha Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. 5-3' Exonuclease No
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. 3-5' Exonuclease (proofreading) No
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Cloned or native Cloned in E. coli
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Frameshift Error Rate 0.00031 errors/bp Technique: Reversion
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Frameshift Error Rate 4E-05 errors/bp Technique: Forward mutational
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Overall Error Rate 0.003 errors/bp Technique: M13mp2 forward mutation assay
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Overall Error Rate 5E-05 errors/bp Technique: Forward mutational
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Nucleotide Substitution Rate 1.3E-06 errors/bp Technique: Reversion
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Nucleotide Substitution Rate 1.1E-05 errors/bp Technique: Forward mutational
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Full length or truncated Full length
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Specific Activity 4.5E+06 units/mg
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Gap Filling Yes
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Cloned or native Insect cells
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 2.38E-05 errors/bp Technique: Reversion (pH 6.1)
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 0.000154 errors/bp Technique: Reversion (pH 7.8)
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 0.000256 errors/bp Technique: Reversion (pH 8.6)
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Full length or truncated Full length
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Specific Activity 3.27E+04 units/mg
Human Pol alpha Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene. Nucleotide Substitution Rate 0.00016 errors/bp
Human Pol alpha DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND. I. LARGE SCALE PURIFICATION OF TERMINAL AND REPLICATIVE DEOXYNUCLEOTIDYL TRANSFERASES. Optimal pH pH 7

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

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It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.