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Polymerase: Human Pol alpha

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This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.

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Polymerase Reference Property Result Context
Human Pol alpha Wong SW1988 3-5' Exonuclease (proofreading) No
Human Pol alpha Copeland WC1992 Incorporation of non-standard nucleotides < 10% Nucleotide analog: Acycloriboucleoside-5'-O-(1-Thiotriphosphate)
Human Pol alpha Copeland WC1992 Incorporation of non-standard nucleotides Unspecified Nucleotide analog: 2',3'-Dideoxyribonucleotide nucleoside triphosphate
Human Pol alpha Copeland WC1992 Cloned or native Native organism
Human Pol alpha Copeland WC1992 Tagged Unspecified
Human Pol alpha Copeland WC1992 Full length or truncated Unspecified
Human Pol alpha Copeland WC1992 KM 1.5uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Activated DNA)
Human Pol alpha Copeland WC1992 KM 1.8uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (activated DNA)
Human Pol alpha Copeland WC1992 KM 110uM Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Running start primer template)
Human Pol alpha Copeland WC1992 KM 1.4uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Running start primer template)
Human Pol alpha Copeland WC1992 KM 2.1uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Running start primer template)
Human Pol alpha Copeland WC1992 Vmax 2 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (Running start primer template)
Human Pol alpha Copeland WC1992 Vmax 0.06 /second Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Running start primer template)
Human Pol alpha Copeland WC1992 Vmax 1.7 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Running start primer template)
Human Pol alpha Copeland WC1992 Application name PCR
Human Pol alpha Copeland WC1992 Kd 120uM Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Steady State (Activated DNA)
Human Pol alpha Copeland WC1992 Kd 650uM Reaction: Nucleotide incorporation; Substrate: TTP analog; Technique: Steady State (Activated DNA)
Human Pol alpha Kamiya H1995 KM 0.2uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Human Pol alpha Kamiya H1995 KM 89uM Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Human Pol alpha Kamiya H1995 Vmax 1.1 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Human Pol alpha Kamiya H1995 Vmax 1.7 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Human Pol alpha Weiser T1991 3-5' Exonuclease (proofreading) No
Human Pol alpha Weiser T1991 5-3' Exonuclease No
Human Pol alpha Weiser T1991 RNase H No
Human Pol alpha Weiser T1991 Processivity 450bp
Human Pol alpha Weiser T1991 Specific Activity 490 units/mg Technique: Polymerase Assay (M13 DNA)
Human Pol alpha Fisher PA1979 Full length or truncated Full length
Human Pol alpha Fisher PA1979 Processivity 11bp
Human Pol alpha Fisher PA1979 KM 44uM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol alpha Fisher PA1979 KM 39uM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol alpha Fisher PA1979 KM 4uM Reaction: Nucleotide incorporation; Substrate: dTTP
Human Pol alpha Fisher PA1979 KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dCTP
Human Pol alpha Fisher PA1979 KM 1.2uM Reaction: Nucleotide incorporation; Substrate: dGTP
Human Pol alpha Fisher PA1979 KM 3uM Reaction: Nucleotide incorporation; Substrate: dATP
Human Pol alpha Fisher PA1979 Nick Extension No
Human Pol alpha Fisher PA1979 Gap Filling Yes
Human Pol alpha Perler FB1996 3-5' Exonuclease (proofreading) No
Human Pol alpha Perler FB1996 5-3' Exonuclease No
Human Pol alpha Perler FB1996 Full length or truncated Full length
Human Pol alpha Boosalis MS1987 KM 3.7uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 KM 4200uM Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 KM 1E+04uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 KM 1E+04uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 KM 35uM Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Gel shift
Human Pol alpha Boosalis MS1987 Vmax 12.9 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 Vmax 3.1 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 Vmax 1.7 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 Vmax 1.7 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Gel shift
Human Pol alpha Boosalis MS1987 Vmax 13 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Gel shift
Human Pol alpha Pavlov YI2004 3-5' Exonuclease (proofreading) No
Human Pol alpha Pavlov YI2004 5-3' Exonuclease No
Human Pol alpha Mo JY1996 3-5' Exonuclease (proofreading) No
Human Pol alpha Mo JY1996 Cloned or native Cloned in E. coli
Human Pol alpha Mo JY1996 Frameshift Error Rate 0.00031 errors/bp Technique: Reversion
Human Pol alpha Mo JY1996 Frameshift Error Rate 4E-05 errors/bp Technique: Forward mutational
Human Pol alpha Mo JY1996 Overall Error Rate 0.003 errors/bp Technique: M13mp2 forward mutation assay
Human Pol alpha Mo JY1996 Overall Error Rate 5E-05 errors/bp Technique: Forward mutational
Human Pol alpha Mo JY1996 Nucleotide Substitution Rate 1.3E-06 errors/bp Technique: Reversion
Human Pol alpha Mo JY1996 Nucleotide Substitution Rate 1.1E-05 errors/bp Technique: Forward mutational
Human Pol alpha Mo JY1996 Full length or truncated Full length
Human Pol alpha Mo JY1996 Specific Activity 4.5E+06 units/mg
Human Pol alpha Mo JY1996 Gap Filling Yes
Human Pol alpha Eckert KA1993 Cloned or native Insect cells
Human Pol alpha Eckert KA1993 Nucleotide Substitution Rate 2.38E-05 errors/bp Technique: Reversion (pH 6.1)
Human Pol alpha Eckert KA1993 Nucleotide Substitution Rate 0.000154 errors/bp Technique: Reversion (pH 7.8)
Human Pol alpha Eckert KA1993 Nucleotide Substitution Rate 0.000256 errors/bp Technique: Reversion (pH 8.6)
Human Pol alpha Eckert KA1993 Full length or truncated Full length
Human Pol alpha Eckert KA1993 Specific Activity 3.27E+04 units/mg
Human Pol alpha Ohashi E2000 Nucleotide Substitution Rate 0.00016 errors/bp
Human Pol alpha YONEDA M1965 Optimal pH pH 7

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.