Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation.

Abstract:

We found that hydroxylation occurs at the C-2 position of adenine by ...

Polymerases:

Topics:

Kinetic Parameters, Nucleotide Incorporation

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. KM 0.2uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. KM 89uM Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. Vmax 1.1 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Human Pol alpha Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. Vmax 1.7 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Klenow fragment Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. KM 0.24uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Klenow fragment Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. KM 1.8uM Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Klenow fragment Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. Vmax 14 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Klenow fragment Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases. Steady-state kinetics of the incorporation. Vmax 23 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State

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