Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III.

Abstract:

Escherichia coli DNA polymerase III holoenzyme is the replicative ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Fidelity, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. 3-5' Exonuclease (proofreading) No
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Cloned or native Cloned in E. coli
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Frameshift Error Rate 0.00031 errors/bp Technique: Reversion
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Frameshift Error Rate 4E-05 errors/bp Technique: Forward mutational
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Overall Error Rate 0.003 errors/bp Technique: M13mp2 forward mutation assay
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Overall Error Rate 5E-05 errors/bp Technique: Forward mutational
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Nucleotide Substitution Rate 1.3E-06 errors/bp Technique: Reversion
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Nucleotide Substitution Rate 1.1E-05 errors/bp Technique: Forward mutational
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Full length or truncated Full length
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Specific Activity 4.5E+06 units/mg
Human Pol alpha Fidelity and error specificity of the alpha catalytic subunit of Escherichia coli DNA polymerase III. Gap Filling Yes

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