Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity.

Abstract:

This paper shows that the protein-primed DNA polymerases encoded by ...

Polymerases:

Topics:

Kinetic Parameters, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Phi29 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. 3-5' Exonuclease (proofreading) Yes
Phi29 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Cloned or native Cloned in E. coli
Phi29 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Full length or truncated Full length
Phi29 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Vmax 2280 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
GA-1 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. 3-5' Exonuclease (proofreading) Yes
GA-1 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Cloned or native Native organism
GA-1 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Full length or truncated Full length
GA-1 Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. kcat 2260 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Nf Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. 3-5' Exonuclease (proofreading) Yes
Nf Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Cloned or native Native organism
Nf Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Full length or truncated Full length
Nf Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. KM 0.6uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Nf Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Vmax 2400 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Nf Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity. Kd 1.5nM Reaction: Nucleotide incorporation; Substrate: dNTPs

Entry validated by: