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Polymerase: Klenow fragment

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Polymerase Reference Property Result Context
Klenow fragment Kong H1993 Processivity 12bp
Klenow fragment Kong H1993 KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Klenow fragment Saiki RK1988 Nucleotide Substitution Rate 8E-05 errors/bp Technique: Sequencing
Klenow fragment Keohavong P1989 Nucleotide Substitution Rate 0.00013 errors/bp Technique: DGGE
Klenow fragment Polesky AH1990 Molecular Weight 6.8E+04 Dalton
Klenow fragment Polesky AH1990 3-5' Exonuclease (proofreading) Yes
Klenow fragment Brautigam CA1999 Other Important Residues Eu3+ at exo site
Klenow fragment Sandin P2009 Methods Featured Fluorescence Spectroscopy
Klenow fragment Sandin P2009 Incorporation of non-standard nucleotides 95% + Nucleotide analog: Fluorescent dNTPs
Klenow fragment Sandin P2009 Vmax 8.4 /minute Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Steady State (dtCoTP fluor analogue); Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C)
Klenow fragment Sandin P2009 Vmax 5.4 /minute Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Steady State (dtCTP fluor analogue); Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C)
Klenow fragment Sandin P2009 Vmax 8.4 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State ; Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C)
Klenow fragment Joyce CM2008 Methods Featured Fluorescence Spectroscopy
Klenow fragment Joyce CM2008 Cloned or native Cloned in E. coli
Klenow fragment Joyce CM2008 Tagged Yes
Klenow fragment Joyce CM2008 Tag Name N-His6
Klenow fragment Joyce CM2008 Full length or truncated Full length
Klenow fragment Joyce CM2008 kcat 140 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Stopped flow (FRET assay of fingers-closing); Experimental conditions: pH (pH 7.5), Mg++ (5mM), Temp (22°C)
Klenow fragment Joyce CM1983 Cloned or native Cloned in E. coli
Klenow fragment Freemont PS1988 Residues Involved in Catalysis of 3-5' Exo D355, E357, D424, D501
Klenow fragment Freemont PS1988 Other Important Residues L361, Q419, R455, F473, Y497, H660
Klenow fragment Beese LS1993 Residues Involved in Catalysis of 3-5' Exo D355, E357, D424, D501
Klenow fragment Beese LS1993 Amino Acids Contacting Template N579, S582, D827, R835
Klenow fragment Beese LS1993 Other Important Residues L361, Q419, R455, F473, Y497, H660, T609, E611, R631, K635, N675, N678
Klenow fragment Derbyshire V1991 3-5' Exonuclease (proofreading) Yes
Klenow fragment Derbyshire V1991 Cloned or native Cloned in E. coli
Klenow fragment Derbyshire V1991 Tagged No
Klenow fragment Derbyshire V1991 Full length or truncated Full length
Klenow fragment Derbyshire V1991 Specific Activity 9000 units/mg Technique: Polymerase assay (poly[d(AT)]
Klenow fragment Derbyshire V1991 KM 0.56uM Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dT)); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
Klenow fragment Derbyshire V1991 kcat 0.09 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dT)); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C)
Klenow fragment Derbyshire V1991 kcat 0.12 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dA)); Experimental conditions: pH (pH 7.5), Mg++ (8mM), Temp (37°C)
Klenow fragment Bebenek K1990 3-5' Exonuclease (proofreading) Yes
Klenow fragment Bebenek K1990 Residues Involved in Catalysis of 3-5' Exo D424, D355, E357, D501
Klenow fragment Bebenek K1990 Frameshift Error Rate 6E-07 errors/bp Technique: M13mp2 forward mutation assay (1 uM dNTP, error rate is upper limit (≤))
Klenow fragment Bebenek K1990 Nucleotide Substitution Rate 5.8E-06 errors/bp Technique: M13mp2 forward mutation assay (1 uM dNTP)
Klenow fragment Derbyshire V1988 Cloned or native Cloned in E. coli
Klenow fragment Derbyshire V1988 Residues Involved in Catalysis of 3-5' Exo D355, E357, D424, D501
Klenow fragment Derbyshire V1988 Tagged No
Klenow fragment Derbyshire V1988 Full length or truncated Full length
Klenow fragment Derbyshire V1988 Other Important Residues 2 Mn ions show metal A and B positions
Klenow fragment Derbyshire V1988 Specific Activity 1.07E+04 units/mg Technique: Polymerase assay (poly[d(AT)]
Klenow fragment Ollis DL1985 Cloned or native Cloned in E. coli
Klenow fragment Ollis DL1985 Tagged No
Klenow fragment Ollis DL1985 Full length or truncated Full length
Klenow fragment Ollis DL1985 Other Important Residues dNMP-Zn interacts with D355, E357, T358, L361, D424, F473, Y497, D501
Klenow fragment Beese LS1991 Residues Involved in Catalysis of 3-5' Exo D355, E357, D424, D501
Klenow fragment Beese LS1991 Other Important Residues T358, L361, Q419, R455, F473, Y497, H660
Klenow fragment Beese LS1993 Amino Acids Contacting NTP R682, Q708, H734, R754, K758, F762, Y766, H881
Klenow fragment Klenow H1970 Molecular Weight 7E+04 Dalton Technique: Gel Filtration
Klenow fragment Klenow H1970 Cloned or native Native organism
Klenow fragment Klenow H1970 5-3' Exonuclease No
Klenow fragment Klenow H1970 Tagged No
Klenow fragment Klenow H1970 Specific Activity 4300 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Klenow H1970 Specific Activity 2 units/mg Technique: Exo activity (arbitrary units) (3H poly d(A-T))
Klenow fragment Lin TC1994 KM 3uM Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Lin TC1994 KM 160uM Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Lin TC1994 kcat 5.8 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Lin TC1994 kcat 7.8 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Brutlag D1972 Vmax 19 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (37°C)
Klenow fragment Brutlag D1972 Vmax 4.8 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (37°C)
Klenow fragment Wang CX2004 kcat 0.02 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ( Substrate: 3'-tailed duplex phosphorothioate substrate)
Klenow fragment Wang CX2004 kcat 0.0017 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 6.5)
Klenow fragment Wang CX2004 kcat 0.0054 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7)
Klenow fragment Wang CX2004 kcat 0.016 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 7.5)
Klenow fragment Wang CX2004 kcat 0.052 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8)
Klenow fragment Wang CX2004 kcat 0.16 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover ; Experimental conditions: pH (pH 8.5)
Klenow fragment Wang CX2004 kcat 0.33 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Single Turnover (Substrate: 3'-tailed duplex all-oxygen substrate)
Klenow fragment Kamiya H1995 KM 0.24uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Klenow fragment Kamiya H1995 KM 1.8uM Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Klenow fragment Kamiya H1995 Vmax 14 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Steady State
Klenow fragment Kamiya H1995 Vmax 23 /minute Reaction: Nucleotide incorporation; Substrate: ATP analog; Technique: Steady State
Klenow fragment Graves SW1998 Processivity 250bp
Klenow fragment Graves SW1998 Vmax 50 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Graves SW1998 Kd 5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment Graves SW1998 Kd 17uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Kiefer JR1998 Polymerase Catalytic Residue Amino Acids Asp705, Asp882, and Glu883
Klenow fragment Kiefer JR1998 3-5' Exonuclease (proofreading) Yes
Klenow fragment Kiefer JR1998 Residues Involved in Catalysis of 3-5' Exo Asp355, Glu357, Asp424, and Asp501
Klenow fragment Kiefer JR1998 Processivity 70bp
Klenow fragment Kiefer JR1998 Specific Activity 5500 units/mg Technique: Polymerase Assay (M13 DNA)
Klenow fragment Kiefer JR1998 Specific Activity 9700 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Kiefer JR1998 KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Kiefer JR1998 KM 1.8nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment Kiefer JR1998 kcat 5.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Bedford E1997 Processivity 20bp
Klenow fragment Bedford E1997 Specific Activity 2200 Other Technique: Polymerase Assay (M13 DNA)
Klenow fragment Minnick DT1996 Molecular Weight 6.8E+04 Dalton
Klenow fragment Minnick DT1996 Cloned or native Native organism
Klenow fragment Minnick DT1996 Tagged No
Klenow fragment Minnick DT1996 Frameshift Error Rate 0.0102 errors/bp Technique: Reversion
Klenow fragment Minnick DT1996 Overall Error Rate 4E-05 errors/bp
Klenow fragment Minnick DT1996 Nucleotide Substitution Rate 0.00036 errors/bp Technique: Reversion
Klenow fragment Minnick DT1996 Nucleotide Substitution Rate 0.0028 errors/bp Technique: Forward mutational
Klenow fragment Minnick DT1996 Full length or truncated Full length
Klenow fragment Minnick DT1996 KM 3.7uM Reaction: Nucleotide incorporation; Substrate: dTTP
Klenow fragment Minnick DT1996 Kd 42nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment Minnick DT1996 kcat 0.3 /second Reaction: Nucleotide incorporation; Substrate: dTTP
Klenow fragment Miller H1997 Template lesions Bypasses Reaction: Nucleotide incorporation; DNA lesion: 8-oxo-dG
Klenow fragment Kukreti P2008 3-5' Exonuclease (proofreading) Yes
Klenow fragment Kukreti P2008 5-3' Exonuclease Unspecified
Klenow fragment Kotlyar AB2005 3-5' Exonuclease (proofreading) Yes
Klenow fragment Kotlyar AB2005 5-3' Exonuclease Unspecified
Klenow fragment Singh K2005 3-5' Exonuclease (proofreading) Yes
Klenow fragment Singh K2005 5-3' Exonuclease Unspecified
Klenow fragment Kotlyar AB2005 3-5' Exonuclease (proofreading) Yes
Klenow fragment Kotlyar AB2005 5-3' Exonuclease No
Klenow fragment Khare V2002 3-5' Exonuclease (proofreading) Yes
Klenow fragment Khare V2002 5-3' Exonuclease No
Klenow fragment Lam WC2002 3-5' Exonuclease (proofreading) Yes
Klenow fragment Lam WC2002 5-3' Exonuclease No
Klenow fragment Terashima I2001 3-5' Exonuclease (proofreading) Yes
Klenow fragment Terashima I2001 5-3' Exonuclease No
Klenow fragment Potapova O2002 Overall Error Rate 0.00038 errors/bp
Klenow fragment Tindall KR1988 3-5' Exonuclease (proofreading) Yes
Klenow fragment Tindall KR1988 Overall Error Rate 0.0044 errors/bp
Klenow fragment Tindall KR1988 Nucleotide Substitution Rate 4E-05 errors/bp
Klenow fragment Tindall KR1988 Full length or truncated Full length
Klenow fragment Papanicolaou C1989 Overall Error Rate 5.8E-05 errors/bp
Klenow fragment Bakhanashvili M1992 Reverse Transcriptase Activity No
Klenow fragment Bakhanashvili M1992 3-5' Exonuclease (proofreading) Yes
Klenow fragment Hogrefe HH2001 Reverse Transcriptase Activity Yes
Klenow fragment Hogrefe HH2001 3-5' Exonuclease (proofreading) Yes
Klenow fragment Hogrefe HH2001 3-5' Exo Specific Activity 28 units/mg
Klenow fragment Hogrefe HH2001 5-3' Exonuclease No
Klenow fragment Hogrefe HH2001 Processivity 7bp
Klenow fragment Hogrefe HH2001 Specific Activity 0.9 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Hogrefe HH2001 Specific Activity 0.6 units/mg Technique: Polymerase Assay (M13 DNA)
Klenow fragment Hogrefe HH2001 KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Hogrefe HH2001 KM 1.8nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment Hogrefe HH2001 Strand Displacement Yes
Klenow fragment Hogrefe HH2001 kcat 310 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Bebenek K1995 Frameshift Error Rate 8E-05 errors/bp Technique: Reversion
Klenow fragment Bebenek K1995 Overall Error Rate 0.0052 errors/bp Technique: M13mp2 forward mutation assay
Klenow fragment Bebenek K1995 Overall Error Rate 7.7E-05 errors/bp Technique: M13mp2 opal codon reversion assay
Klenow fragment Setlow P1972 Molecular Weight 7.6E+04 Dalton
Klenow fragment Setlow P1972 3-5' Exonuclease (proofreading) Yes
Klenow fragment Setlow P1972 5-3' Exonuclease No
Klenow fragment Setlow P1972 Molecular Weight 7.6E+04 Dalton Technique: SDS-acrylamide gel electrophoresis
Klenow fragment Setlow P1972 3-5' Exonuclease (proofreading) Yes
Klenow fragment Setlow P1972 5-3' Exonuclease No
Klenow fragment Setlow P1972 Specific Activity 1.534E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Setlow P1972 Extinction Coefficient 0.93 at 278nm

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.